AI Article Synopsis
- Researchers created helical aromatic oligoamide foldamers with anionic side chains that resemble DNA's shape and charge, aiming to study their binding interactions with the chromosomal protein Sac7d, which also binds to DNA.
- A variety of scientific techniques, including Surface Plasmon Resonance and Nuclear Magnetic Resonance, were used to analyze how well these foldamers interact with Sac7d, showing they bind more effectively than a similar length DNA duplex.
- Structural analysis via crystallography indicated that these foldamers have a unique binding arrangement with Sac7d, allowing them to attach without causing the usual kinking seen in DNA.
Article Abstract
Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked.
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| http://dx.doi.org/10.1002/anie.202422958 | DOI Listing |
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