Purpose: To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.
Methods: The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury. We divided sixty-four rats into four groups: the Injury group (Control group), the AAM group, the sPL group, and the sPL-AAM group. Our study compared the endometrial thickness, gland count, and fibrotic area recovery in rats at 6 days and 18 days post-treatment. Immunohistochemistry was utilized to assess the expressions of CD34 and ANG. Additionally, we used ELISA to detect the levels of IL-6 and TNF-α, while Western Blot was employed to compare the expressions of CK19, Integrin β3, and TGF-β1. One month after the treatment, we evaluated and compared the pregnancy recovery among the groups.
Results: Compared to the Injury group, the sPL-AAM group demonstrated enhanced endometrial regeneration in rats at both 6 days and 18 days post-treatment, resulting in a favorable pregnancy outcome. This was achieved by promoting angiogenesis, suppressing the inflammatory response, and reducing fibrosis. The observed effects were superior to those of the sPL group alone. While sPL, when administered alone, showed some degree of endometrial restoration at 6 days post-treatment, its efficacy was diminished at 18 days post-treatment. The impact of AAM alone appeared inconspicuous compared to the injury group. This suggests that sPL serves as the primary agent in facilitating endometrial repair, while AAM functions as a carrier to extend the duration of sPL's effectiveness.
Conclusion: sPL-AAM can release effective cytokines, repair endometrial damage in rats, enhance endometrial receptivity, and ultimately improve pregnancy outcomes.
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http://dx.doi.org/10.2147/JIR.S483446 | DOI Listing |
J Inflamm Res
December 2024
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
Purpose: To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.
Methods: The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury.
Environ Toxicol Pharmacol
December 2024
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, Al-Azhar University, Assiut Branch, Assiut, 71524, Egypt. Electronic address:
Massylaea vermiculata snails are a significant gastropod pest in Egypt, threatening agriculture. Due to increasing concerns about conventional pesticides, it is imperative to find effective alternatives that are less harmful. We assessed the molluscicidal activity of abamectin, methoxyfenozide, and spinosad using the leaf-dipping method in vitro and the effect of LC of these compounds on biochemical aspects and protein electrophoresis.
View Article and Find Full Text PDFExpert Opin Drug Saf
December 2024
Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University.
Eur Endod J
December 2024
Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Naples, Italy.
Objective: To evaluate the effect of a newly developed MTA-based material and two antibacterial-enhanced MTAs as pulp capping materials in immature permanent dental elements underwent full pulpotomy.
Methods: The present animal study included 20 Wistar albino rats that, after full pulpotomy, were randomly divided into 4 groups receiving different MTA formulations as pulp capping materials: conventional MTA, Tricalcium aluminate (TCA)- free MTA, and MTA enhanced with metronidazole or doxycycline. Histopathological assessments were carried out at 7- and 28-days post-treatment to evaluate dentinal bridge formation, inflammatory reactions, pulp tissue necrosis and internal resorption.
PLoS One
December 2024
Postgraduate Program in Biophotonics Medicine Applied to Health Sciences, Universidade Nove de Julho, Sao Paulo, Brazil.
Objective: This study aims to evaluate the efficacy of photodynamic therapy as an adjunct to conventional endodontic treatment in patients with apical periodontitis and fistulas. In this study, a fistula is characterized as a pathological conduit originating from the infected region at the root apex of the tooth, traversing the oral mucosa, and extending to the external surface of the gingiva. This pathological condition frequently complicates the management of endodontic infections, thereby necessitating the evaluation of supplementary therapeutic interventions.
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