A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 994
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3134
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Rolling Circle Amplification Integrating with Exonuclease-III-Assisted Color Reaction for Sensitive Telomerase Activity Analysis. | LitMetric

AI Article Synopsis

  • Telomerase activation contributes to cancer cell growth by allowing cells to bypass normal aging, making its detection vital for diagnosing and treating cancer.
  • A new detection platform utilizes exonuclease-III for enhanced signal amplification and employs a colorimetric reaction that is simple, fast, and sensitive.
  • The platform demonstrates high sensitivity and specificity when tested on real samples, showing potential for use in clinical settings and cancer treatment development.

Article Abstract

Telomerase activation can lead to the escape from cell senescence and immortalization, playing a crucial role in the growth and proliferation of cancer cells. Therefore, the detection of telomerase activity is essential for cancer diagnosis and treatment. Herein, we develop a novel ultrasensitive and visually detectable platform. By incorporation of exonuclease-III (Exo-III), this platform achieves dual signal amplification of rolling circle amplification products. Additionally, the colorimetric analysis of 3,3',5,5'-tetramethylbiphenyl (TMB) chromogenic reaction system provides this approach with unique advantages such as simplicity, speediness, and sensitivity. The detection platform exhibits high sensitivity and specificity in actual sample testing, which aligns closely with results obtained using commercial kits. Moreover, it offers ease-of-use through visual determination by the naked eyes. This finding indicates that our proposed sensing method performs satisfactorily in detecting telomerase in real biological samples. Henceforth, we believe that this sensing platform holds great potential for clinical diagnosis and anticancer drug development.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656203PMC
http://dx.doi.org/10.1021/acsomega.4c03839DOI Listing

Publication Analysis

Top Keywords

rolling circle
8
circle amplification
8
telomerase activity
8
amplification integrating
4
integrating exonuclease-iii-assisted
4
exonuclease-iii-assisted color
4
color reaction
4
reaction sensitive
4
telomerase
4
sensitive telomerase
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: