Significance: Neuronal dendritic spines are central elements for memory and learning. Their morphology correlates with synaptic strength and is a proxy for function. Classic light microscopy cannot resolve spine morphology well, and techniques with higher resolution (electron microscopy and super-resolution light microscopy) typically do not provide spine data in large fields of view, e.g., along entire dendrites. Therefore, it remains unclear if spine types are organized on mesoscopic scales, despite their undisputed importance for understanding the brain.
Aim: Recently, it was shown that the distribution of spine type is dendrite-specific in the turtle cortex, suggesting a mesoscopic organization, but leaving the question open if such a dendrite specificity also exists in mammals. Here, we determine if such a difference in spine-type distribution among dendrites also exists in the mouse brain.
Approach: We used super-resolution stimulated emission depletion microscopy of complete dendrites and advanced morphological analysis in three dimensions to decipher morphological differences of spines on different dendrites.
Results: We found that spines of different shapes decorate different dendrites of the same neuron to a varying extent. Significant differences among the dendrites are apparent, based on spine classes as well as based on quantitative descriptors, such as spine length or head size.
Conclusions: Our findings may indicate that it is an evolutionarily conserved principle that individual dendrites have distinct distributions of spine types hinting at individual roles.
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http://dx.doi.org/10.1117/1.NPh.12.1.015001 | DOI Listing |
Front Bioeng Biotechnol
December 2024
Department of Orthopaedics, Peking University Third Hospital, Beijing, China.
Objective: To confirm the effect of surgery on spinal column biomechanics and to provide theoretical support for the advantages and disadvantages of different surgical methods and their clinical efficacy.
Methods: 33 continuous patients with no significant difference in risk factors related to the mechanical complications were enrolled in this retrospective study. Sagittal parameters were measured in the pre-, post-operative and following-up lateral radiograph of spine.
Neurophotonics
January 2025
Saarland University, Molecular Imaging, Center for Integrative Physiology and Molecular Medicine, Homburg, Germany.
Significance: Neuronal dendritic spines are central elements for memory and learning. Their morphology correlates with synaptic strength and is a proxy for function. Classic light microscopy cannot resolve spine morphology well, and techniques with higher resolution (electron microscopy and super-resolution light microscopy) typically do not provide spine data in large fields of view, e.
View Article and Find Full Text PDFGlobal Spine J
December 2024
Department of Spine Surgery, Ganga Hospital, Coimbatore, India.
Study Design: Observational comparative study.
Objective: To study the role of magnetic resonance spectroscopy (MRS) and T2 relaxometry (T2r) as imaging biomarkers for identifying early lumbar disc degeneration.
Methods: We evaluated 236 discs in normal volunteers and 215 discs in low back pain (LBP) patients by MRS and T2r to document the molecular spectra of various metabolites as well as disc hydration and collagen content, respectively.
J Imaging Inform Med
December 2024
Department of Radiology, Research Institute of Radiological Science and Center for Clinical Imaging Data Science, Yonsei University College of Medicine, 50-1 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Korea.
The accurate and early detection of vertebral metastases is crucial for improving patient outcomes. Although deep-learning models have shown potential in this area, their lack of prediction reliability and robustness limits their clinical utility. To address these challenges, we propose a novel technique called Ensemble Monte Carlo Dropout (EMCD) for uncertainty quantification (UQ), which combines the Monte Carlo dropout and deep ensembles.
View Article and Find Full Text PDFLancet Neurol
January 2025
Spinal Cord Injury Center, Balgrist University Hospital, University of Zurich, Zurich, Switzerland.
Background: Spinal cord injury results in permanent neurological impairment and disability due to the absence of spontaneous regeneration. NG101, a recombinant human antibody, neutralises the neurite growth-inhibiting protein Nogo-A, promoting neural repair and motor recovery in animal models of spinal cord injury. We aimed to evaluate the efficacy of intrathecal NG101 on recovery in patients with acute cervical traumatic spinal cord injury.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!