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The role of inflammasome dysregulation in obstructive and non-obstructive azoospermia: a comparative molecular analysis of blood, tissue, and seminal plasma. | LitMetric

AI Article Synopsis

  • - This study investigates the role of inflammasomes in azoospermia, focusing on gene expression differences in key inflammasome components between obstructive (OA) and non-obstructive azoospermia (NOA) patients.
  • - Results showed higher inflammasome gene expression and elevated levels of IL-1β and IL-18 in NOA patients compared to OA and normospermic (NS) individuals, suggesting chronic inflammation's contribution to azoospermia.
  • - The findings indicate a potential for developing diagnostic tools to distinguish between azoospermia types based on inflammasome gene expression profiles, highlighting the importance of chronic inflammation in NOA.

Article Abstract

Background: To address knowledge gaps, this study aimed to investigate the involvement of inflammasomes in the etiology of azoospermia. This study focused on the gene expression of key inflammasome components, including , and .

Methods: We analyzed gene expression in blood and testicular tissue from patients with obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Additionally, we compared IL-1β and IL-18 expression levels in seminal plasma samples using the Enzyme-Linked Immunosorbent Assay (ELISA) method. For comparison, blood samples from normospermic (NS) individuals were also genetically evaluated.

Results: Our results indicated significantly higher gene expression of inflammasome components in NOA patients than those in OA patients either in blood or in testicular tissue. Both azoospermic groups exhibited higher mRNA levels of inflammasome genes comparing with those from blood samples of NS men. Seminal plasma samples showed significantly increased levels of IL-1β and IL-18 in NOA patients compared to men with OA. The ROC curve analysis indicated strong and significant predictive power of and gene expression profiles between NOA vs. NS patients and NOA vs. OA.

Conclusions: Our findings highlight the role of hidden chronic inflammation in azoospermia, particularly within the NOA group. This study provides a foundation for further detailed research, which could aid in the development of diagnostic panels to differentiate between various azoospermic groups.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659152PMC
http://dx.doi.org/10.3389/fimmu.2024.1507885DOI Listing

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