The exact mechanisms and key functional molecules involved in skin ageing remain largely unknown. Studies linking the expression of messenger RNAs (mRNAs) and small noncoding RNAs (sncRNAs) to skin ageing are limited. In this study, we performed RNA sequencing to assess the effects of ageing on the expression of mRNAs and sncRNAs in rat skin. Our results revealed that 241 mRNAs, 109 microRNAs (miRNAs), 20 piwi-interacting RNAs (piRNAs), 45 small nucleolar RNAs (snoRNAs), and 7 small nuclear RNAs (snRNAs) were significantly differentially expressed in the skin of aged rats compared to their younger counterparts. Histological validation using RT-qPCR further verified the significant differential expression of 13 mRNAs, 7 miRNAs, 2 piRNAs, 15 snoRNAs, and 1 snRNA. Additionally, several sncRNAs showed differential expression across various tissues, suggesting that they may have broad correlations with ageing. After establishing cellular senescence in skin fibroblasts, we identified 4 mRNAs, 4 miRNAs, and 10 snoRNAs that may mediate skin ageing by modulating fibroblast senescence. Notably, overexpression or knockdown of some differentially expressed RNAs in fibroblasts influenced cellular senescence, indicating that these RNAs could play an important role in the skin ageing process. These findings highlight their potential significance for future treatments of age-related skin disorders.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mad.2024.112022 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetic Causal Association Between Skin Microbiota and Biological Aging: Evidence From a Mendelian Randomization Analysis.
J Cosmet Dermatol
January 2025
Department of Neonatology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, China.
Background: The skin microbiota, a complex community of microorganisms residing on the skin, plays a crucial role in maintaining skin health and overall homeostasis. Recent research has suggested that alterations in the composition and function of the skin microbiota may influence the aging process. However, the causal relationships between specific skin microbiota and biological aging remain unclear.
View Article and Find Full Text PDFRecent advances in biomarkers for senescence: Bridging basic research to clinic.
Geriatr Gerontol Int
January 2025
Department of Advanced Senotherapeutics and Department of Cardiovascular Biology and Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
In this review, we review the current status of biomarkers for aging and possible perspectives on anti-aging or rejuvenation from the standpoint of biomarkers. Aging is observed in all cells and organs, and we focused on research into senescence in the skin, musculoskeletal system, immune system, and cardiovascular system. Commonly used biomarkers include SA-βgal, cell-cycle markers, senescence-associated secretory phenotype (SASP) factors, damage-associated molecular patterns (DAMPs), and DNA-damage-related markers.
View Article and Find Full Text PDFAccelerated aging modulates the toxicological properties of the diazo tattoo pigment PO13.
Sci Rep
January 2025
Univ. Grenoble Alpes, CEA, CNRS, Grenoble INP, SyMMES, Grenoble, F-38000, SyMMES, France.
Pigment particles used in tattooing may exert long terms effect by releasing diffusible degradation products. In the present work, aqueous suspensions of the organic orange diazo pigment PO13 were aged by exposure to simulated sunlight at 40 °C. The morphology and the surface charge of PO13 particles were barely modified upon aging, but primary particles were released by de-agglomeration.
View Article and Find Full Text PDFInnovative analytical methodology for skin anti-aging compounds discovery from plant extracts: Integration of High-Performance Thin-Layer Chromatography-in vitro spectrophotometry bioassays with multivariate modeling and molecular docking.
J Chromatogr A
December 2024
Department of Analytical Chemistry, University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11158 Belgrade, Serbia. Electronic address:
Skin aging, characterized by reduced elasticity, wrinkles, and changes in pigmentation, presents significant challenges in the cosmetics industry. Identifying compounds that can help mitigate these effects is crucial to developing effective anti-aging treatments and improving skin health. An advanced analytical approach for identifying skin anti-aging compounds within complex natural mixtures must be developed to achieve this.
View Article and Find Full Text PDFThe involvement of Elf5 in regulating keratinocyte proliferation and differentiation processes in skin.
PLoS One
January 2025
School of Science and Technology, Nottingham Trent University, Nottingham, United Kingdom.
Article Synopsis
- Skin and hair development involves complex gene regulation to ensure proper growth and maintenance.
- Elf5 is identified as a key transcription factor that influences keratinocyte proliferation and differentiation in skin and hair follicles.
- Research on Elf5 could pave the way for new treatments in stem cell research, regenerative medicine, and age-related skin issues.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!