AI Article Synopsis
- * The focus was on understanding how the enzyme's efficiency changes based on the molar ratios of the oligosaccharide donor, sialoglycopeptide (SGP), to the CD acceptors with different glucose arrangements.
- * We also analyzed the production of CD-based oligosaccharide clusters formed through the action of Endo-M on these acceptors.
Article Abstract
We investigated the transglycosylation reaction of two types of oligosaccharide acceptors, i.e., β-cyclodextrin (CD) derivatives 1 and 2 conjugated with multiple glucose (Glc) units, catalyzed by endo-β-N-acetyl-glucosaminidase from Mucor hiemalis (Endo-M) using the oligosaccharide donor sialoglycopeptide (SGP). The acceptor specificity of the enzyme transglycosylation of 1 and 2 having seven Glc moieties within small nanoscale spatial regions on the β-CDs was investigated on the basis of the effect of the molar ratios of SGP to acceptors 1 or 2 with different spatial configurations on the transglycosylation behavior. The formation of the corresponding CD-based oligosaccharide clusters from Endo-M was also evaluated.
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| http://dx.doi.org/10.1016/j.carres.2024.109352 | DOI Listing |
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