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Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS. | LitMetric

Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.

Methods Mol Biol

Simons Centre for the Study of Living Machines, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India.

Published: December 2024

AI Article Synopsis

Article Abstract

Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations.

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Source
http://dx.doi.org/10.1007/978-1-0716-4280-1_4DOI Listing

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