Background: MicroRNAs (miRNAs) have emerged as an essential regulator of the cell fate commitment of neural stem/progenitor cells (NPCs), although the impacts of certain miRNAs on NPCs remain vague. The aim of this study is to investigate the regulatory effects of on the cell fate commitment of NPCs.
Methods: We investigated the impact of on the proliferation and differentiation capacities of primary NPCs by manipulating the expression of using specific mimics and inhibitors. The effects of on NPCs was confirmed through stereotactic injection of antagonists to the brains of mice at postnatal day 1 (P1).
Results: The expression levels of kept increasing in the differentiation process of NPCs and . Perturbation of 's function showed that inhibited NPCs' proliferation and promoted embryonic NPCs to differentiate more favorably to the glial lineage. We then validated the anti-proliferation and pro-glial roles of using NPCs isolated from P1 mouse brains. study further showed enlarged NPCs pools and inhibited gliogenesis in the brains of P1 mice after animals received antagomir-185-5p.
Conclusion: Our study suggests as an important regulator for the proliferation and glial fate commitment of NPCs.
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| http://dx.doi.org/10.3389/fcell.2024.1510746 | DOI Listing |
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