Background: Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues.
Methods: Single-cell RNA sequencing data were used to create a detailed single-cell ecological landscape of DM and DFU. Enrichment analysis identified pathways involved in cellular subpopulations, and pseudo-time analysis inferred cell development processes. A gene regulatory network explored the role of transcription factors in DFU progression, and a potential herbal drug-target gene interaction network was constructed.
Results: In the DFU group, immune cells were activated, with notable changes in several subpopulations. ATP5E was significantly overexpressed in Naive T cells, fibroblasts, endothelial cells, and CD8 T cells in DM patients. Specific immune cell subsets, such as Naive T_RGCC, CTL_TYROBP_CL4, Mac_SLC40A1, and M1_CCL3L1, likely contribute to DFU formation through overactivation and proliferation, leading to tissue damage and ulcer exacerbation. Key genes TPP1, TLR4, and RIPK2 were identified, and 88 active ingredients in the herbal drug-target network showed strong correlations with these targets. Herbs like Angelica dahurica, Angelica sinensis, Boswellia carterii, liquorice, myrrh, and Semen armeniacae amarae were included.
Conclusions: This study offers insights into DM and DFU cytology. T cells in DFU are activated, attacking normal tissues and worsening tissue damage. The ATP5E gene may be related to the ecological remodeling of DM, and TPP1, TLR4, and RIPK2 are potential targets for DFU treatment.
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http://dx.doi.org/10.1186/s40001-024-02179-7 | DOI Listing |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657181 | PMC |
Diabetes Res Clin Pract
December 2024
Health Sciences University Gazi Yaşargil Training and Research Hospital, Nursing Services, Diyarbakır, Turkey.
Background And Aim: Given the significance of pain management in the context of diabetic foot ulcer (DFU) care, particularly in terms of ensuring patient adherence to treatment regimens, this study was undertaken to ascertain the impact of cold washing on pain and comfort levels during diabetic foot care.
Methods: A single-blind randomized controlled trial was conducted in the chronic wound care clinic of a teaching and research hospital in the southeastern region of Turkey. The sample consisted of 68 patients, with 34 assigned to the cold application group and 34 assigned to the control group.
Bioorg Chem
December 2024
College of Pharmacy and Chemistry & Chemical Engineering, Taizhou University, 93 East Jichuan Road, Hailing District, Taizhou, Jiangsu 225300, China. Electronic address:
Single treatment methods for wound infections caused by drug-resistant bacteria often fail to simultaneously achieve high antimicrobial efficacy and wound healing efficiency. Therefore, it is crucial to develop a small molecule prodrug that can achieve both goals, in this context, we have engineered a green-light-activated nitric oxide photocage/photodynamic therapeutic prodrug, designed to release NO, which not only potentiates antimicrobial efficacy but also facilitates collagen accumulation at the wound interface, thereby expediting the wound healing process. Additionally, it mitigates tissue inflammation by suppressing the NF-κB signaling pathway.
View Article and Find Full Text PDFInt J Low Extrem Wounds
December 2024
Australian Centre for Health Services Innovation, School of Public Health and Social Work, Queensland University of Technology, Brisbane, Australia.
This study aimed to investigate the levels of adherence to wearing therapeutic footwear, and the factors associated, among people at high-risk of diabetes-related foot ulcers (DFUs) in Jordan. This was a secondary analysis of data from a multi-centre cross-sectional study of participants at high-risk of DFU in Jordan who had therapeutic footwear. Participants had socio-demographic, health, limb, and psychosocial variables collected, plus self-reported their proportion of adherence time wearing therapeutic footwear on an average day (excluding sleeping time) using a visual analogue scale.
View Article and Find Full Text PDFEur J Med Res
December 2024
Department of Bone and Joint Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China.
Background: Diabetes mellitus (DM) can cause severe complications, including diabetic foot ulcers (DFU). There is a significant gap in understanding the single-cell ecological atlas of DM and DFU tissues.
Methods: Single-cell RNA sequencing data were used to create a detailed single-cell ecological landscape of DM and DFU.
Int J Low Extrem Wounds
December 2024
Department of Clinical laboratory, Xi'an Medical University Second Affiliated Hospital, Xi'an, Shaanxi, China.
Diabetic Foot Ulcer (DFU) is a common and challenging complication of diabetes mellitus, associated with high recurrence, hospitalization, disability, and mortality rates. The Systemic Immune Inflammatory Index (SIRI) based on complete blood counts has been shown to correlate with several diseases but has not been widely studied in DFU.This study aimed to explore the relationship between SIRI and the presence of DFU in individuals with diabetes using data from the National Health and Nutrition Examination Survey (NHANES).
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