This study investigated the mechanism by which ginsenoside Rg_(1 )attenuates hypoxia/reoxygenation(H/R) injury in HL-1 cardiomyocytes by inhibiting the acetylation of ATP synthase subunit alpha(ATP5A1) through silent information regulator 3(SIRT3). In this study, an H/R injury model was constructed by hypoxia for 6 h and reoxygenation for 2 h in HL-1 cardiomyocytes. First, the optimal effective concentration of ginsenoside Rg_1 was determined using a cell viability assay kit. Then, lactate dehydrogenase(LDH) leakage was measured using a microplate method to evaluate the protective effect of ginsenoside Rg_1 against H/R injury in HL-1 cardiomyocytes. Western blot was further used to detect SIRT3 expression and the acetylation level of mitochondrial proteins in cardiomyocytes. ATP content was measured using a luciferase assay. Immunoprecipitation was used to detect the acetylation level of ATP5A1. The oxygen consumption rate(OCR) was measured using the Seahorse XFp analyzer. Flow cytometry was used to assess cell apoptosis to explore the specific mechanism. The results showed that compared with the control group, the model group had a significant increase in LDH leakage, a decrease in SIRT3 expression, a significant increase in the acetylation levels of mitochondrial proteins and ATP5A1, a decrease in OCR and ATP content, and an increase in cell apoptosis rate. Compared with the model group, the ginsenoside Rg_1 group showed a significant decrease in LDH leakage, an increase in SIRT3 expression, a significant decrease in the acetylation levels of mitochondrial proteins and ATP5A1, an increase in OCR and ATP content, and a decrease in cell apoptosis rate. Compared with the ginsenoside Rg_1 group, the ginsenoside Rg_1 + si SIRT3 group showed a significant increase in LDH leakage, a decrease in SIRT3 expression, a significant increase in the acetylation levels of mitochondrial proteins and ATP5A1, a decrease in OCR and ATP content, and an increase in cell apoptosis rate. In conclusion, ginsenoside Rg_1 alleviates H/R injury in HL-1 cells by improving mitochondrial respiratory function through SIRT3-mediated inhibition of ATP5A1 acetylation.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.19540/j.cnki.cjcmm.20240620.401 | DOI Listing |
Ecotoxicol Environ Saf
December 2024
Department of Pharmacy, Chaohu Hospital of Anhui Medical University, China. Electronic address:
This study investigates the protective effect of ginsenoside Rg1 against manganese (Mn)-induced hepatotoxicity, highlighting its role as a PPAR-γ activator and its impact on TLR4/MyD88/MAPK pathway. Manganese induces liver damage through mechanisms involving oxidative stress and inflammation. Rg1, a principal bioactive compound of ginseng, significantly alleviates Mn-induced liver injury.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
November 2024
School of Life Sciences, Beijing University of Chinese Medicine Beijing 100029, China.
Based on the differences in the protective effects of fresh Panax ginseng and its processed products on myocardial ischemia in mice, this study identified the advantageous aspects of fresh P. ginseng. By using network pharmacology combined with cell model validation, the molecular mechanisms of fresh P.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
November 2024
School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China Beijing Key Laboratory of Traditional Chinese Medicine Collateral Disease Theory Research Beijing 100069, China.
This study rapidly identified and quantified the chemical components of the Wuzhuyu Decoction nanophase(WZYD-N) and suspension phase(WZYD-S) using ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry(UPLC-QQQ-MS/MS). Based on preliminary pharmacodynamic experiments and network pharmacology analysis, the differential anti-inflammatory and analgesic activities of WZYD-N and WZYD-S were explored to understand their pharmacodynamic basis. WZYD-N and WZYD-S were separated by differential centrifugation-dialysis, and their particle size, Zeta potential, PDI, and morphology were characterized by dynamic light scattering and transmission electron microscopy.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
October 2024
Institute of Basic Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences Beijing 100091, China National Clinical Research Center for Chinese Medicine Cardiology Beijing 100091, China.
This study investigated the mechanism by which ginsenoside Rg_(1 )attenuates hypoxia/reoxygenation(H/R) injury in HL-1 cardiomyocytes by inhibiting the acetylation of ATP synthase subunit alpha(ATP5A1) through silent information regulator 3(SIRT3). In this study, an H/R injury model was constructed by hypoxia for 6 h and reoxygenation for 2 h in HL-1 cardiomyocytes. First, the optimal effective concentration of ginsenoside Rg_1 was determined using a cell viability assay kit.
View Article and Find Full Text PDFJ Pharm Biomed Anal
December 2024
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China. Electronic address:
Shenhua compound, composed of ginseng, hawthorn and sophora flower, has been shown to improve hyperlipidemia. However, the main ingredients, their metabolic pathways in vivo, and pharmacokinetic characteristics. In this study, ultra-high-performance liquid chromatography coupled with electrospray ionization quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF) was used to qualitatively analyze the main ingredients in ethanol extract of Shenhua compound and to investigate the metabolites in serum, bile, feces, and urine of rats following oral administration.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!