Regulation of PILS genes by bZIP transcription factor TGA7 in tomato plant growth.

Auxin plays a pivotal role in plant growth regulation. The PIN-FORMED (PIN) proteins facilitate long-distance polar auxin transport, whereas the recently identified PIN-LIKES (PILS) proteins regulate intracellular auxin homeostasis. However, the auxin transport mechanisms in horticultural crops remain largely unexplored. Here, we identified and characterized PILS genes in tomato (Solanum lycopersicum). Promoter analysis revealed enrichment in TGA[C/T]G motifs, suggesting transcriptional regulation by TGA factors in the bZIP family. Subcellular localization studies confirmed that all tomato PILS proteins localize in the endoplasmic reticulum. PILS2 exhibited the highest expression across examined tissues, and its close homologue PILS6 showed a similar but less pronounced expression pattern. Silencing PILS2 significantly inhibited shoot and root growth. Phylogenetic and expression analyses identified the homologs of Arabidopsis TGA1, TGA3, TGA4, and TGA7 in tomato genome, with tomato TGA7 showing higher expression in roots. Notably, silencing tomato TGA7, but not TGA1, TGA3, or TGA4, strongly impaired shoot and root growth. Molecular assays demonstrated that TGA7 directly binds to the PILS2 promoter to activate its transcription. These findings uncover a TGA7-PILS2 regulatory module that governs plant growth and offer new insights into the function and regulation of PILS genes in tomato.