Isolation, purification, structural characterization, and antitumor activity of Gynura divaricata polysaccharides.

Int J Biol Macromol

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Hunan Provincial Key Laboratory of the Research and Development of Novel, Pharmaceutical Preparations, the "Double-First Class" Applicatio Characteristic, Discipline of Hunan Province (Pharmaceutical Science), Changsha Medical University, Changsha 410219, China. Electronic address:

Published: December 2024

In this study, a water-soluble, homogeneous polysaccharide, GDPs-1, was isolated from Gynura divaricata and purified. It was subsequently subjected to structural characterization and biological activity assessment. Structural characterization revealed that GDPs-1 was mainly composed of glucose, galactose, and arabinose, with a molecular weight of 55.1 kDa. Its main structural backbone was →[4)-α-D-Glcp-(1] → 4)-α-D-Glcp-(1 → 4)-α-D-Glcp-(1 → [4)-β-D-Galp-(1]→, with two additional branched structures. In vitro experiments demonstrated that GDPs-1 induced cancer cell apoptosis, caused cell cycle blockade, increased the Bax/Bcl-2 protein ratio, attenuated epithelial-mesenchymal transition, and activated caspase-3 protein in carcinoma cells, thereby exerting antitumor effects on tumor cells without affecting the growth of normal cells. Therefore, GDPs-1 may be further explored as a novel medicine to treat lung and liver cancer.

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Source
http://dx.doi.org/10.1016/j.ijbiomac.2024.138928DOI Listing

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