Multi-responsive biosensor prepared based on MXene and PDEA-HRP binary architecture films for HO detection and logic gate construction.

The quantitative detection of HO is of great significance for preventing the occurrence of diseases. In this work, an electrochemical biosensor for detecting HO was constructed through a step-by-step modification method. The PDEA-HRP/MXene/PG biosensor (PDEA = poly(N,N-dimethyl acrylamide), HRP = horseradish peroxidase, PG = pyrolytic graphite) was prepared with two-dimensional metal carbide (MXene) nano materials as the inner layer and PDEA-HRP hydrogel as the outer layer for the detection of HO. Due to the excellent conductivity and biocompatibility of MXene materials, the prepared PDEA-HRP/MXene/PG biosensors have high sensitivity, wide linear range, and good repeatability. The results indicated that under optimal conditions, the prepared biosensor can detect HO concentration within a linear range of 0.04 mM ∼ 1.80 mM, with the detection limit of 1.08 × 10 mM (S/N = 3). The detection effect was good in actual samples. In addition, based on the switching properties of PDEA-HRP hydrogel under different conditions, combined with the characteristics of MXene nanomaterials. This study also constructed several biomolecule electrocatalytic logic gate systems, including binary 5-Input/5-Output logic gate network, 2-to-4 decoder, and a ternary AND logic gates.