A Novel System for Simultaneous Real-Time Determination of N-Enriched Ammonia, Hydroxylamine, Nitrite, and Nitrate.

Anal Chem

Institute of Carbon Neutrality, Sino-French Institute for Earth System Science, College of Urban and Environmental Sciences, Peking University, Beijing 100091, China.

Published: December 2024

Ammonium (NH), hydroxylamine (NHOH), nitrite (NO), and nitrate (NO) account for the most important reactive nitrogen (N) species in the N cycle, playing a key role in N elimination and N retention, as well as the production of nitrogenous trace gases. However, it is still challenging to fulfill simultaneous real-time determination of all four N compounds enriched in N. This study successfully established a novel system by coupling an utomatic imultaneous ample reparation unit to a embrane nlet ass pectrometer (4n-ASSP-MIMS) for rapid online N fraction analysis of all four key compounds in the N cycle. The limit of detection (LOD) was 20.22, 0.38, 0.17, and 0.25 μM for NH, NHOH, NO, and NO, respectively, and relative error for N fraction determination was within 5% when N enrichment was higher than 5 atom %. This 4n-ASSP-MIMS system provides a first online analytical tool to capture the real-time isotopic signals during biogeochemical transformations of NH, NHOH, NO, and NO, using the N tracing technique. Application of this system will dramatically advance our understanding of mechanisms involved in the N cycle.

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http://dx.doi.org/10.1021/acs.analchem.4c05467DOI Listing

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