Imaging mass spectrometry (IMS) is a technique for simultaneously acquiring the expression and distribution of molecules on the surface of a sample, and it plays a crucial role in spatial omics research. In IMS, the time cost and instrument load required for large data sets must be considered, as IMS typically involves tens of thousands of pixels or more. In this study, we developed a high-resolution method for IMS data reconstruction using a window-based Adversarial Autoencoder (AAE) method. We acquired IMS data from partial cerebellum regions of mice with a pitch size of 75 μm and then down-sampled the data to a pitch size of 150 μm, selecting 22 / peak intensity values per pixel. We established an AAE model to generate three pixels from the surrounding nine pixels within a window to reconstruct the image data at a pitch size of 75 μm. Compared with two alternative interpolation methods, Bilinear and Bicubic interpolation, our window-based AAE model demonstrated superior performance on image evaluation metrics for the validation data sets. A similar model was constructed for larger mouse kidney tissues, where the AAE model achieved high image evaluation metrics. Our method is expected to be valuable for IMS measurements of large animal organs across extensive areas.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1021/jasms.4c00372 | DOI Listing |
An ecological niche is a field in a landscape of affordances, rich in information inviting its inhabitants to develop functionality and effectiveness of their behavior. This idea means that, in sports like soccer, the playing area encapsulates an ecological niche, replete with affordances inviting collective and individual technical-tactical actions, contextualized with associated psychological and physical demands. To examine the co-adaptive relationships framing players' actions in their ecological niche, the present study employed a crossover design with repeated measures to compare the players' transactions within 11 vs.
View Article and Find Full Text PDFPhys Med Biol
December 2024
Department of Biomedical Engineering, University of California Davis, 451 East Health Science Drive, Davis, California, 95616, UNITED STATES.
Position-sensitive silicon photomultipliers (PS-SiPMs) are promising photodetectors for ultra-high spatial resolution small-animal positron emission tomography (PET) scanners. This paper evaluated the performance of the latest generation of linearly-graded SiPMs (LG-SiPMs), a type of PS-SiPM, for ultra-high spatial resolution PET applications using LYSO arrays from two vendors. Approach: Two dual-ended readout detectors were developed by coupling LG-SiPMs to both ends of the two LYSO arrays.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
December 2024
Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.
Materials (Basel)
November 2024
Department of Furniture Design, Faculty of Forestry and Wood Technology, Poznań University of Life Sciences, 60-637 Poznań, Poland.
Many factors affect screw withdrawal resistance (SWR), including screw size, embedment depth, the pre-drilled hole's diameter, dimensional accuracy, and the furniture pieces' material properties being joined. While prior research has extensively examined the influence of these factors, this study aimed to explore a neglected factor: how drill bit wear impacts pilot hole quality and subsequent SWR. The experimental setup included pinewood samples with pre-drilled 5 mm diameter blind pilot holes with a depth of 45 mm.
View Article and Find Full Text PDFGait Posture
December 2024
School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, NSW 2308, Australia. Electronic address:
Background: Posture of the upper neck is considered by some to contribute to neck pain and headache. Infrared reflectors and cameras permit non-invasive three-dimensional (3D) evaluation of head and neck range of motion but have not been used to examine the relative pose (position and orientation) of the head, upper and lower neck.
Research Question: Can the relative 3D pose of the head, upper and lower neck regions of adult humans be non-invasively measured and perturbated while recording the characteristics of neck or head pain?
Methods: An optical motion capture system was used in a repeat measure descriptive analysis study.
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!