AI Article Synopsis
- - The study examines how chromosomal abnormalities in embryos affect their development patterns, specifically focusing on morphokinetic changes that could help in selecting viable embryos for implantation.
- - Researchers analyzed data from 939 fertility cycles, involving 2876 biopsied embryos, and identified significant morphokinetic parameters that indicate whether an embryo is euploid (normal) or aneuploid (abnormal).
- - Findings reveal that fragmentation and deletion of chromosomes, particularly types 20 and 22, lead to marked delays in key developmental stages, providing valuable insights for improving embryo selection processes in fertility treatments.
Article Abstract
Background: The change of morphokinetic pattern in aneuploid embryos will facilitate the non-invasive selection of euploid embryos. In this study, we investigated the impact of different chromosomal abnormalities on the morphokinetic patterns of embryonic development.
Methods: Our cohort includes 939 time-lapse preimplantation genetic testing cycles performed between January 2019 and July 2022 at a single academic fertility center, with a total of 2876 biopsied blastocysts. Intracytoplasmic sperm injection, blastocyst culture, trophectoderm biopsy, time-lapse monitoring, and next-generation sequencing were performed.
Results: After adjusting for patient- and cycle-related factors, ix morphokinetic parameters (t5, P = 0.006; t8, P = 0.048; tSB, P < 0.001; tB,P < 0.001; t5-t2, P = 0.004; tB-tSB, P < 0.001) were significant in multilevel mixed-effects logistic regression model analysis for morphokinetic parameters to predict euploid or aneuploid embryos. None of the patient- or cycle-related factors systematically affected any morphokinetic parameter. Morphokinetic parameters of late cleavage and blastocyst stages in embryos with chromosome fragment deletion (t4 to t8, tB, t5-t2, tB-tSB, ECC2, ECC3, s2, P < 0.05) or duplication (t4, t5, tSB, tB, t5-t2, P < 0.05) were prolonged, and the morphokinetic parameters of the blastocyst stage in monosomic embryos (tSB, tB, tB-tSB, P < 0.01) were prolonged. Partial or complete chromosome 20 or 22 deletion can cause significant delays in multiple parameters of cleavage and blastocyst stages (from t4 to tB, P < 0.05).
Conclusions: Our study found that different chromosomal abnormalities have different effects on the morphokinetic parameters. Significant delays in morphokinetic parameters at different stages were found in fragment-mutated embryos and monosomic embryos. This can provide insights into the pre-implantation development pattern of aneuploid embryos and help non-invasive embryo selection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11647804 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2024.e40686 | DOI Listing |
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