A protein corona (PC) is formed and maintained on the surface of any nanoparticle (NP) introduced into biological media. The full PC is formed by a hard and soft corona, and the latter determines the nature of the interaction of NPs with cells and the body's liquids. Nanomedicines are becoming increasingly important in modern health services, making information about the composition of PCs on the surface of NPs critically important for "managing" the behavior of nano-objects in the body. Currently, only a few studies report on the composition of the complete PC, since the isolation and preservation of the soft corona on the surface of the NP is extremely difficult. Recently, we proposed for the first time a photomodification method to fix PCs on the lipid surface of composite NPs, along with their isolation and purification. In this work, using tandem mass spectrometry, we successively determined the composition of the hard and full corona on the lipid surface of composite NPs, and we also identified the composition of the soft corona. To test the method, we changed the composition of the medium whose proteins formed the soft corona, and we found changes in its composition.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3390/nano14231976 | DOI Listing |
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11643909 | PMC |
Publication Analysis
Top Keywords
Similar Publications
The Photomodification Method Allows for Determining the Composition of the Full and Soft Protein Corona on the Lipid Surface of Composite Nanoparticles.
Nanomaterials (Basel)
December 2024
Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia.
A protein corona (PC) is formed and maintained on the surface of any nanoparticle (NP) introduced into biological media. The full PC is formed by a hard and soft corona, and the latter determines the nature of the interaction of NPs with cells and the body's liquids. Nanomedicines are becoming increasingly important in modern health services, making information about the composition of PCs on the surface of NPs critically important for "managing" the behavior of nano-objects in the body.
View Article and Find Full Text PDFInterpolyelectrolyte complexes of a biguanide cationic polyelectrolyte: formation of core/corona nanoparticles with double-hydrophilic diblock polyanion.
Soft Matter
December 2024
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 2030, 128 40, Prague 2, Czech Republic.
Article Synopsis
Extracellular vesicles of different cellular origin feature distinct biomolecular corona dynamics.
Nanoscale Horiz
December 2024
Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.
Initially observed on synthetic nanoparticles, the existence of biomolecular corona and its role in determining nanoparticle identity and function are now beginning to be acknowledged in biogenic nanoparticles, particularly in extracellular vesicles - membrane-enclosed nanoparticle shuttling proteins, nucleic acids, and metabolites which are released by cells for physiological and pathological communication - we developed a methodology based on fluorescence correlation spectroscopy to track biomolecular corona formation on extracellular vesicles derived from human red blood cells and amniotic membrane mesenchymal stromal cells when these vesicles are dispersed in human plasma. The methodology allows for tracking corona dynamics under physiological conditions. Results evidence that the two extracellular vesicle populations feature distinct corona dynamics.
View Article and Find Full Text PDFThe role of patient-specific variables in protein corona formation and therapeutic efficacy in nanomedicine.
J Nanobiotechnology
November 2024
Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM, USA.
Despite their potential, the adoption of nanotechnology in therapeutics remains limited, with only around eighty nanomedicines approved in the past 30 years. This disparity is partly due to the "one-size-fits-all" approach in medical design, which often overlooks patient-specific variables such as biological sex, genetic ancestry, disease state, environment, and age that influence nanoparticle behavior. Nanoparticles (NPs) must be transported through systemic, microenvironmental, and cellular barriers that vary across heterogeneous patient populations.
View Article and Find Full Text PDFSAXS, DLS, and MD studies of the Rg/Rh ratio for swollen and collapsed dendrimers.
J Chem Phys
November 2024
Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia.
The radius of gyration, Rg, and the hydrodynamic radius, Rh, are the main experimental parameters that characterize the size of linear and branched macromolecules. In the case of dendrimers in solution, the ratio Rg/Rh, depending on the global conformation, varies from 1 (for a Gaussian soft sphere) to 3/5 (for a hard sphere). However, for high-generation dendrimers, this ratio may be less than the limiting value for a hard sphere.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!