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The bHLH Transcription Factor PhbHLH121 Regulates Response to Iron Deficiency in . | LitMetric

The bHLH Transcription Factor PhbHLH121 Regulates Response to Iron Deficiency in .

Plants (Basel)

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Published: December 2024

AI Article Synopsis

  • Iron (Fe) is crucial for plant growth, but low availability in soils leads to Fe deficiency issues in agriculture.
  • The study focuses on the transcription factor PhbHLH121 in petunias, which is downregulated when plants face Fe shortages, and its loss-of-function enhances Fe deficiency tolerance.
  • Additionally, PhbHLH121 impacts gene expression related to Fe uptake and storage, indicating it plays a significant role in managing the plant's response to Fe scarcity.

Article Abstract

Iron (Fe) is an essential micronutrient for plants. Due to the low Fe bioavailability in cultivated soils, Fe deficiency is a widespread agricultural problem. In this study, we present the functional characterization of a petunia () basic-helix-loop-helix transcription factor PhbHLH121 in response to Fe shortage. Real-time PCR revealed that the expression of in petunia roots and shoots was downregulated under Fe-limited conditions. CRISPR/Cas9-edited mutant plants were generated to investigate the functions of PhbHLH121 in petunia. Loss-of-function of PhbHLH121 enhanced petunia tolerance to Fe deficiency. Further investigations revealed that the expression level of several structural genes involved in Fe uptake in petunia, such as and , was higher in mutants compared to that in wild-type under Fe-limited conditions, and the expression level of several genes involved in Fe storage and Fe transport, such as , and , was lower in mutants compared to that in wild-type under Fe-deficient conditions. Yeast one-hybrid assays revealed that PhbHLH121 binds to the G-box element in the promoter of genes involved in Fe homeostasis. Yeast two-hybrid assays revealed that PhbHLH121 interacts with petunia bHLH IVc proteins. Taken together, PhbHLH121 plays an important role in the Fe deficiency response in petunia.

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Source
http://dx.doi.org/10.3390/plants13233429DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11644503PMC

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