AI Article Synopsis

  • - Ex vivo fluorescence laser scanning microscopy (FCM) enables real-time imaging of fresh tissue samples without traditional slide preparation, and this study specifically investigates its application in evaluating biopsy samples from endoscopic procedures for lung lesions and lymph nodes.
  • - The study involved 32 patients, showing that the FCM Vivascope 2500 could accurately assess sample adequacy and malignancy, with a perfect agreement noted between its evaluations and final histological results.
  • - The findings suggest that FCM could significantly improve the speed of diagnostic information during endoscopic biopsies, potentially enhancing patient management by reducing the time needed to diagnose lung cancer.

Article Abstract

: Ex vivo fluorescence laser scanning microscopes (FCMs) allow digital tissue imaging directly from fresh, unfixed specimens without the need for conventional histological slide preparation. To date, no data have been reported on the use of FCMs in the endoscopy suite for immediate evaluation of endoscopic ultrasound (EUS)/endobronchial ultrasound (EBUS) fine needle aspiration/biopsy (FNA-B) specimens of lung lesions and/or mediastinal lymph nodes. : The aim of this study was to evaluate the performance of the FCM Vivascope 2500 (Vivascope, Munich, Germany) in providing real-time adequacy assessment and diagnostic information on the digital images of fresh unprocessed EUS/EBUS FNA-B specimens and to compare it with the corresponding final histological sections of formalin-fixed and paraffin-embedded cell blocks. : Thirty-two patients (50% male; 71 ± 8 years old) were enrolled between May 2023 and June 2024. In 28/32 (87.5%) patients, samples were defined as adequate at Vivascope evaluation, and in 20/28 (71.4%) patients, samples were classified as malignant. At final cytohistological evaluation, 87.5% of specimens were defined as adequate and 20/28 (71.4%) were diagnosed as malignant. There was perfect agreement between the Vivascope assessment of adequacy and the final cytohistological assessment on the same specimen (k Cohen 1). From a diagnostic point of view, perfect agreement was found between the two techniques in the identification of malignant neoplasms (k Cohen 1). : The use of FCM could provide rapid information on both the adequacy and malignancy of the sample obtained during EBUS tissue acquisition (EBUS-TA), with minimal or no preparation and without destroying or losing the tissue. This technique allows for obtaining representative material in EBUS/EUS for lung cancer staging and is expected to change the turnaround time from biopsy sampling to diagnostic characterization of the tumor, ultimately improving patient management both at diagnosis and at restaging in follow up.

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Source
http://dx.doi.org/10.3390/cancers16234015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11640265PMC

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