Oxathiapiprolin, a novel fungicide targeting oxysterol-binding proteins (OSBP), has proven to be effective against various oomycete diseases, but its effects on pathogens remain underexplored. In this study, we demonstrated that oxathiapiprolin inhibits mycelium growth, sporangium production, zoospore release, and oospore production in , with 50% effective concentrations ranging from 0.000153 to 0.011681 μg mL. Additionally, in vivo tests confirmed its efficacy in controlling litchi downy blight. Our study demonstrated that oxathiapiprolin disrupts cellular ultrastructure and plasma membrane integrity, affects stress sensitivity, inhibits sterol transport, increases autophagy levels, reduces lipid droplet content, and induces lipid metabolism disorders. Gene expression analysis revealed that oxathiapiprolin affects the expression of (ORP1 gene of ), with docking simulations further confirming its interaction with the PlORP1 (ORP1 protein of ) protein. Ultimately, this cascade of events led to pathogen death. This paper reveals the antioomycete mechanism of oxathiapiprolin against .
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http://dx.doi.org/10.1021/acs.jafc.4c09639 | DOI Listing |
J Agric Food Chem
December 2024
School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.
Oxathiapiprolin, a novel fungicide targeting oxysterol-binding proteins (OSBP), has proven to be effective against various oomycete diseases, but its effects on pathogens remain underexplored. In this study, we demonstrated that oxathiapiprolin inhibits mycelium growth, sporangium production, zoospore release, and oospore production in , with 50% effective concentrations ranging from 0.000153 to 0.
View Article and Find Full Text PDFPestic Biochem Physiol
June 2024
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China; Department of Plant Pathology, College of Plant Protection, China Agricultural University, 2 Yuanmingyuanxi Road, Beijing 100193, China. Electronic address:
The phytopathogenic oomycete Phytophthora litchii is the culprit behind the devastating disease known as "litchi downy blight", which causes large losses in litchi production. Although fluopimomide exhibits strong inhibitory efficacy against P. litchii, the exact mechanism of resistance is still unknown.
View Article and Find Full Text PDFVirulence
December 2024
Hainan Yazhou Bay Seed Laboratory, College of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Sanya, China.
Autophagy is an intracellular degradation process that is important for the development and pathogenicity of phytopathogenic fungi and for the defence response of plants. However, the molecular mechanisms underlying autophagy in the pathogenicity of the plant pathogenic oomycete , the causal agent of litchi downy blight, have not been well characterized. In this study, the autophagy-related protein ATG2 homolog, PlATG2, was identified and characterized using a CRISPR/Cas9-mediated gene replacement strategy in .
View Article and Find Full Text PDFMicroorganisms
January 2024
Department of Plant Pathology/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.
Litchi is a fruit of significant commercial value; however, its quality and yield are hindered by downy blight disease caused by . In this study, volatile organic compounds (VOCs) from TJGA-19 were investigated for their antifungal effects and studied in vitro and in planta for the suppression of litchi downy blight disease in litchi leaves and fruits. The growth of was inhibited by VOCs produced by TJGA-19 cultivated on autoclaved wheat seeds for durations of 10, 20, or 30 days.
View Article and Find Full Text PDFJ Agric Food Chem
January 2024
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, 3 Taicheng Road, Yangling 712100, Shaanxi China.
In this study, we determined the sensitivity of 148 isolates to cyazofamid, yielding a mean EC value of 0.0091 ± 0.0028 μg/mL.
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