Cortical microtubules act as a template to organize nano-scale patterning of exocytosis.

Targeting of exocytosis enables cellular morphogenesis, motility and polarized transport, yet relatively little is known about the targeting mechanisms in cellular systems. Here we show that the SEC/MUNC protein KEULE is a dynamic marker for individual secretory events and employ it as a live cell probe, that together with high-precision image analysis of thousands of events, reveal that cortical microtubule arrays act as two-dimensional templates that pattern exocytosis at the nano-scale in higher plant cells. This mechanism is distinct from previously described mechanisms involving motor-driven transport and defines ordered and adjacent linear domains where secretory events are higher and lower than expected, effectively redistributing exocytosis over most of the cell membrane. In addition, analysis of KEULE kinetics revealed distinct phases of assembly/disassembly that are differentially sensitive to experimental treatments that reduce exocytosis, revealing SEC/MUNC dynamics as a versatile and information rich read-out of exocytosis .