An Escherichia coli serogroup O55 strain produced heat-labile enterotoxin only, which exerted unusual effects on cell cultures; it caused elongation of CHO and HeLa cells but no changes in Y-1 cells. Injection of this substance, designated LT', into mouse foot pad and rabbit skin caused a well-expressed necrotic effect beside the LT-like activity. LT' showed no cytotoxic effect and failed to produce mouse lung oedema. The strain was not haemolytic. According to Sephadex G-100 fractionation, the LT' had a high molecular weight. The LT' and the necrotic activities could not be separated by fractionation. Neutralization experiments suggested an antigenic relationship between LT and LT'. The antigenic deficiency of LT' was closely related to the common antigenic component of LT and choleragen. The necrotic effect caused by crude LT' was neutralized only by the homologous serum.

Download full-text PDF

Source

Publication Analysis

Top Keywords

lt'
8
escherichia coli
8
coli serogroup
8
serogroup o55
8
o55 strain
8
altered heart-labile
4
heart-labile enterotoxin
4
enterotoxin lt'
4
lt' produced
4
produced escherichia
4

Similar Publications

Figurate erythema in 11 Eastern European sphynx cats.

Vet Dermatol

June 2024

Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.

Background: In humans, figurate erythema (FE) represents a heterogenous group of dermatoses with circular or serpiginous erythematous skin lesions; FE has not been reported in cats.

Objectives: To report clinical and histological characteristics and outcomes of FE in sphynx cats from Baltic sea-bordering countries.

Animals: Eleven client-owned sphynx cats with FE.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!