Activated carbon and anthraquinone-2,6-disulfonate as electron shuttles for enhancing carbon and nitrogen removal from simultaneous methanogenesis, Feammox and denitrification system.

Anthraquinone-2,6-disulfonate (AQDS) and activated carbon (AC) were employed as exogenous electron shuttles (ESs) for enhancing the performance of an integrated simultaneous methanogenesis, Feammox, and denitrification (SMFD) system treating fish sludge. The addition of AQDS and AC led to an increased total nitrogen removal efficiency by 30.2 % and 66.5 %, an increased total chemical oxygen demand removal efficiency by 9.5 % and 24.5 %, and an improved methane yield by 5.2 % and 12.6 %, respectively. Regarding nitrogen removal, AQDS mainly facilitated NH-N oxidation into NO-N via Feammox, while AC facilitated both Feammox and denitrification. Regarding carbon removal, both ESs promoted the hydrolysis-acidification process via stimulating dissimilatory iron reduction and established direct interspecies electron transfer (DIET) between methanogens and syntrophic bacteria. Microbial analysis confirmed the enrichment of iron-reducing bacteria, denitrifiers, DIET-related methanogens and syntrophic partners in the presence of ESs. The study provides an ESs-assisted strategy for enhancing simultaneous nitrogen and carbon removal from high-strength wastewater.