Design of novel dual-emitting ratiometric fluorescence system based on FMT-CDs and MnO NS for sensitive isoniazid detection in urine sample.

Spectrochim Acta A Mol Biomol Spectrosc

Institute of Pharmaceutical Analysis, School of Pharmacy, Lanzhou University, Lanzhou 730030, China. Electronic address:

Published: December 2024

AI Article Synopsis

  • A ratiometric fluorescence probe utilizing carbon dots and MnO nanosheets was developed to detect isoniazid (INH) using a novel method.
  • The probe works by the interaction between the oxidase-like activity of MnO NS and non-fluorescent o-phenylenediamine, producing a fluorescent compound that is quenched in the presence of INH.
  • This method demonstrated high sensitivity for INH detection in human urine samples, achieving a limit of detection as low as 0.16 μM.

Article Abstract

In this work, a simple and sensitive ratiometric fluorescence probe to detect isoniazid (INH) was developed on the basis of carbon dots and MnO nanosheets (MnO NS). Nitrogen-doped carbon dots (FMT-CDs) were synthesized by microwave method using formononetin (FMT) in ammonia water. The oxidase-like activity of MnO NS was utilized to oxidize non-fluorescent o-phenylenediamine (OPD) to 2, 3-diaminophenazine (DAP) with orange fluorescence. It was demonstrated that DAP could effectively quench the fluorescence of FMT-CDs through the inner filtering effect (IFE). Due to the reducing nature of INH, MnO NS was decomposed and thus lost its oxidase-like activity after the addition of INH. Therefore, the production of DAP was reduced, and the fluorescence intensity was decreased, while IFE on FMT-CDs was weakened and the fluorescence of FMT-CDs was recovered. Based on the above principle, a sensitive method for the detection of INH was established based on the fluorescence intensity ratio of the two fluorescent substances, FMT-CDs and DAP, and was successfully applied to the detection of INH in human urine samples. The limit of detection (LOD) could reach to 0.16 μM in human urine samples.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2024.125586DOI Listing

Publication Analysis

Top Keywords

ratiometric fluorescence
8
carbon dots
8
oxidase-like activity
8
fluorescence fmt-cds
8
fluorescence intensity
8
detection inh
8
human urine
8
urine samples
8
fluorescence
7
fmt-cds
6

Similar Publications

In this report, we successfully engineered a novel probe based on an acceptor-donor-acceptor (A-D-A) architecture featuring dicyanovinyl-substituted thieno[3,2-]thiophene, termed DCVTT. The designed probe self-assembles into luminous nanoparticles (DCVTT NPs) upon introducing mixed aqueous solutions. These fluorescent nanostructures served as a ratiometric probe for detecting cyanide (CN) ions in aqueous-based environments, owing to the robust Intramolecular Charge Transfer (ICT) characteristics of DCVTT.

View Article and Find Full Text PDF

Bovine serum albumin (BSA) is one of the most abundant proteins in serum, and its high-throughput detection is still one of the current challenges. Nitrogen‑phosphorus co-doped carbon dots (CDs) were synthesized by a hydrothermal method. Adenosine monophosphate (AMP) was used as a precursor for the synthesis of CDs, providing the required carbon, nitrogen and phosphorus sources for the CDs.

View Article and Find Full Text PDF

A simply designed quasi-ratiometric fluorescence probe for the visual and on-site detection of levofloxacin (LVF) residues in milk and fish sample.

Talanta

December 2024

College of Chemistry and Chemical Engineering, Henan Key Laboratory of Function-Oriented Porous Materials, Luoyang Normal University, Luoyang, 471934, China.

An europium metal organic framework (Eu-DBPA-Phen) was synthesized using 2,5-dibromoterephthalic acid (HDBPA) and 1-10-phenanthroline (Phen) as ligands. A straightforwardc quasi-ratiometric fluorescence probe was then developed for the detection of levofloxacin (LVF) by the simplistic combination of red-emitting Eu-DBPA-Phen and the inherent blue auto-fluorescence of the target. The probe exhibits the advantages of wide linear range (0.

View Article and Find Full Text PDF

Peroxynitrite (ONOO) and viscosity are critical indicators of lysosome functionality, intimately linked to numerous diseases' pathophysiological processes. Hence, creating reliable analytical techniques to observe fluctuations in lysosomal ONOO and viscosity is highly important. This study presents the development of a novel naphthalimide-based fluorescent probe, Nap-Cy, specifically designed to target lysosomes and simultaneously detect both ONOO and viscosity.

View Article and Find Full Text PDF

Copper-based fluorescent nanozyme used to construct a ratiometric sensor for visual detection of thiophanate methyl.

Talanta

December 2024

Key Laboratory of Catalytic Conversion and Clean Energy in Universities of Shandong Province, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu City, 273165, Shandong, China. Electronic address:

Although nanozyme has shown great potential in designing fluorescent assays for pesticide residue, most of them are based on single emission, thus affecting the detection accuracy. Herein, a copper-based fluorescent nanozyme (Cu-BH) synthesized with dual-ligand, integrating fluorescence and oxidase-mimic into one spherical nanomaterial, was used firstly to establish a ratiometric approach for visual detection of thiophanate methyl (TM). Cu-BH possesses excellent oxidase-like activities, triggering the oxidation of colorless o-phenylenediamine (OPD) into yellow luminescent products (oxOPD, λem = 564 nm).

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: