Quantification of lysosomal labile Zn and monitoring of Zn efflux using a small-molecule-protein hybrid fluorescent probe.

Yuyin Du Toshiyuki Kowada EunHye Sung Rong Liu Andrei Soloviev Toshitaka Matsui Shin Mizukami

J Inorg Biochem

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan; Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan; Department of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aramaki Aza-Aoba, Aoba-ku, Sendai, Miyagi 980-8578, Japan. Electronic address:

Published: March 2025

Lysosomal labile Zn levels have been unclear. By targeting a small-molecule fluorescent Zn probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn efflux from the lysosomes to cytosol upon TRPMLs activation.

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http://dx.doi.org/10.1016/j.jinorgbio.2024.112811	DOI Listing

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