Isolation of 5-hydroxy-7-methoxy-8-O-β-D-glucopyranosyl-2S-flavanone from a new source, : characterization and anticancer activity.

This study explores the anticancer properties of , a plant indigenous to the Western Ghats of India. We isolated and characterised 5-hydroxy-7-methoxy-8-O-β-D-glucopyranosyl-2S-flavanone from , previously unreported in this plant, using preparative HPLC, NMR, and HRMS techniques. The compound's molecular structure and purity were confirmed, and its biological activity was evaluated on the MDA-MB-231 breast cancer cell line using the MTT assay. The compound exhibited notable cytotoxic effects with an IC value of 155.21 ± 1.59 sM. This work validates the therapeutic capabilities of and contributes to ongoing research on natural substances for anticancer drug development. The results suggest that could be a promising source of bioactive compounds, opening new possibilities for cancer therapy approaches.