AI Article Synopsis
- Phagocytic cells play a crucial role in the immune response during viral infections, and researchers often study their impact by comparing infected animals with and without these cells.
- Clodronate liposomes are an effective and straightforward method for depleting phagocytes in animal models, allowing for tailored depletion either systemically or locally.
- The study focuses on using clodronate liposomes to deplete blood-contacting phagocytes and examine their interactions with the La Crosse virus in a simplified model of viral presence in the blood.
Article Abstract
During viral infection, phagocytic cells participate in numerous immunological processes. A common approach to elucidate the specific contributions of phagocytic cells is through direct comparison of viral pathogenesis of phagocyte-depleted and phagocyte-intact animals. Clodronate liposomes are a versatile and simple means of depleting phagocytes in any animal model. Selection of clodronate liposome route of delivery can yield systemic or local phagocyte depletion as needed. Here we describe the application of clodronate liposome-mediated depletion of blood-contacting phagocytes to investigate their interactions with La Crosse virus (or other bunyaviruses) in a reductionist model of viremia.
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| http://dx.doi.org/10.1007/978-1-0716-4338-9_21 | DOI Listing |
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