AI Article Synopsis
- Bunyaviruses, transmitted primarily by mosquitoes, trigger the mosquito's innate immune response, particularly through RNA interference (RNAi) pathways.
- Despite known interactions, there is a lack of comprehensive data on how these viruses are specifically targeted by the mosquito immune response.
- This chapter outlines protocols for testing whether a specific protein can inhibit bunyavirus infections in mosquito cells, as well as methods for examining the effects of overexpressing or mutating mosquito-encoded proteins.
Article Abstract
Viruses of the class Bunyaviricetes are often transmitted by arthropods, including mosquitoes. The innate immune response in mosquitoes comprises several pathways, including sequence-specific degradation through RNA interference (RNAi). It is known that bunyavirus infections are targeted by the innate immune response in mosquitoes and derived cells; however, detailed information is often still missing. In this chapter, we describe a detailed protocol to determine if a protein of interest acts antiviral against bunyavirus infection in mosquito-derived cells. In line, we also describe a detailed protocol to investigate the effect of overexpression of a mosquito-encoded protein or analysis of mutant proteins on bunyavirus infection.
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