Background: Oral squamous cell carcinoma (OSCC) is a multifactorial disease. Despite continuing research, the role and prevalence of human papillomavirus (HPV) in oral carcinogenesis initiation and progression remain elusive.
Aim: This study aimed to detect high-risk HPV 16 and 18 DNA in archival tissue specimens of OSCC using multiplex polymerase chain reaction (PCR) and E6 and E7 DNA in the samples positive for HPV 16 and HPV 18 DNA.
Methods: Detection of viral DNA in 90 samples of OSCC was achieved using multiplex PCR with primers specific for HPV 16 and 18. Positive samples were further subjected to multiplex PCR to detect the presence of viral E6 and E7 DNA.
Results: Among the 90 samples evaluated, 23 (25.6%) were positive for HPV 16 DNA and two (2.2%) for HPV 18 DNA. None showed the presence of both strains in the same sample. Among the 23 samples positive for HPV 16, 17 (73.9%) showed combined expression of E6 and E7 DNA, six (23.1%) expressed E6 DNA alone, and none expressed E7 DNA. Both the samples positive for HPV 18 showed the expression of E7 DNA alone.
Conclusion: The present study establishes the existence of a subset of patients within the Indian subpopulation that harbor the oncogenic strains of HPV. This contributes to the global pool of data and reinforces the need for future research to delve into the role of prophylactic vaccination targeting oncogenic HPV.
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| http://dx.doi.org/10.7759/cureus.73427 | DOI Listing |
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