This study introduces an innovative bioinspired hydrogel scaffold tailored to facilitate the in-situ integration of hybrid nanoflowers (HNFs) into the sensing interface, thereby establishing a versatile dual-mode platform for the sensitive profiling of acetylcholinesterase (AChE) inhibitors, a pivotal aspect in the pursuit of Alzheimer's disease therapeutics. Mimicking the tenacious anchoring of natural tree roots, our design employs magnetic bead imprinting with Strep-Tactin-coated magnetic beads (STMBs) to shape the hydrogel, which is then complemented by the integration of AChE-specific aptamers. This configuration creates a stable and biomimetic environment that nurtures HNF growth, thereby enhancing the binding integrity of HNFs with sensing interfaces. The platform's dual-mode detection capability, integrating both colorimetric and electrochemical sensing, is demonstrated through the effective evaluation of galantamine's inhibitory potency, with IC values that highlight its therapeutic potential. The hydrogel's exceptional reusability, maintaining over 95% of its initial activity after multiple uses, and its long-term stability, retaining 91% of its initial performance, further highlight its practicality and cost-effectiveness. In summary, this bioinspired hydrogel scaffold offers a novel, efficient, and dependable biosensing strategy for HNF-based biosensors, showing great potential for broad applications in medical diagnostics and advanced biosensing technologies.
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http://dx.doi.org/10.1016/j.bios.2024.117032 | DOI Listing |
Soft Matter
December 2024
Hefei National Research Center for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, P. R. China.
Bioinspired supramolecular adhesives have been recently emerging as novel functional materials, which have shown a wide range of applications in wearable sensors and tissue engineering such as tissue adhesives and wound dressings. In this review, we summarize and discuss two main types of biologically inspired supramolecular adhesives from adhesive proteins and nucleic acids. The widely studied catechol-based adhesives, that originated from adhesive proteins of marine organisms such as mussels, and recently emerging nucleobase-containing supramolecular adhesives are both introduced and discussed.
View Article and Find Full Text PDFACS Appl Mater Interfaces
December 2024
Research Institute for Biomaterials, Tech Institute for Advanced Materials Bioinspired Biomedical Materials & Devices Center, College of Materials Science and Engineering, Jiangsu Collaborative Innovation Center for Advanced Inorganic Function Composites, Suqian Advanced Materials Industry Technology Innovation Center, Nanjing Tech University, Nanjing 211800, China.
Transdermal insulin delivery in a painless, convenient, and on-demand way remains a long-standing challenge. A variety of smart microneedles (MNs) fabricated by glucose-responsive phenylboronic acid hydrogels have been previously developed to provide painless and autonomous insulin release in response to a glucose level change. However, like the majority of MNs, their transdermal delivery efficiency was still relatively low compared to that with subcutaneous injection.
View Article and Find Full Text PDFBiosens Bioelectron
December 2024
College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, PR China. Electronic address:
This study introduces an innovative bioinspired hydrogel scaffold tailored to facilitate the in-situ integration of hybrid nanoflowers (HNFs) into the sensing interface, thereby establishing a versatile dual-mode platform for the sensitive profiling of acetylcholinesterase (AChE) inhibitors, a pivotal aspect in the pursuit of Alzheimer's disease therapeutics. Mimicking the tenacious anchoring of natural tree roots, our design employs magnetic bead imprinting with Strep-Tactin-coated magnetic beads (STMBs) to shape the hydrogel, which is then complemented by the integration of AChE-specific aptamers. This configuration creates a stable and biomimetic environment that nurtures HNF growth, thereby enhancing the binding integrity of HNFs with sensing interfaces.
View Article and Find Full Text PDFBioact Mater
March 2025
Department of Anatomy, Engineering Research Center for Organ Intelligent Biological Manufacturing of Chongqing, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Third Military Medical University, Chongqing, 400038, China.
Nat Commun
December 2024
Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China.
While hydrogel-based flexible sensors find extensive applications in fields such as medicine and robotics, their performance can be hindered by the rapid evaporation of water, leading to diminished sensitivity and mechanical durability. Despite the exploration of some effective solutions, such as introducing organic solvents, electrolytes, and elastomer composites, these approaches still suffer from problems including diminished conductivity, interface misalignment, and insufficient protection under dynamic conditions. Inspired by cell membrane structures, we developed an adaptive lipid-integrated bilayer coating (ALIBC) to enhance water retention in hydrogel-based sensors.
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