Brain development during the lifespan of cynomolgus monkeys.

The use of stereotaxic brain templates in automatic neuroimage analysis has become the predominant technology. However, in the context of experiment studies involving cynomolgus monkeys, there is a lack of adequate brain templates. This gap poses a challenge to effectively utilize cynomolgus monkeys in neuroscience research. Considering the dynamic changes in structural morphology and functional maturity during development and aging, particularly post-birth, improving the accuracy of image data analysis necessitates the construction of a series of stereotaxic brain templates spanning all age groups. Therefore, this study developed a comprehensive set of stereotaxic templates for development cynomolgus brain with fine substructure delineations, including T1WI MRI structural template, tissue probability map (TPM), [F]FDG PET functional template aligned in a standardized space. This template set enables precise localization and quantification of cynomolgus monkey brain tissue across different developmental stages. Additionally, it could also be used for automatic individualized substructure segmentation. By automatically generating multiple precision subregion segmentations within individual spaces, it preserves the integrity of individual data with high precision and repeatability. Our constructed cynomolgus template sets can further facilitate the accurate study of brain structure and glucose metabolism function in non-human being studies.