Background: Hundreds of miRNAs have been reported to be dysregulated in Parkinson's disease (PD), providing valuable assistance in improving its diagnosis. The purpose of this study was to analyze the expression and diagnostic value of miR-19b-3p in PD patients, as well as its relationship with inflammatory factors.
Methods: We recruited 50 PD patients and 50 healthy age- and gender-matched controls and collected demographic data and biochemical parameters from both groups. RT-qPCR was used to detect miR-19b-3p levels in serum. ELISA was used to detect serum levels of TNF-α and IL-1β. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic value of miR-19b-3p in PD patients.
Results: Serum miR-19b-3p expression was downregulated in PD patients compared with healthy controls. A negative correlation was found between miR-19b-3p levels and the MDS-UPDRS score in PD patients (r = -0.686, p < 0.01). In ROC curve analysis, the area under the curve of miR-19b-3p for prediction of PD was 0.779. In PD patients, serum miR-19b-3p levels were negatively correlated with serum IL-1β (r = -0.556, p < 0.01) and TNF-α levels (r = -0.592, p < 0.01).
Conclusions: Serum miR-19b-3p might serve as a diagnostic and predictive biomarker for PD. The association detected between miR-19b-3p and two common markers of inflammation (IL-1β and TNF-α) may suggest a role for miR-19b-3p in PD-associated neuroinflammation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7754/Clin.Lab.2024.240639 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Electroacupuncture improves pulmonary function by reducing inflammatory reaction via inhibiting miR-19b-3p to regulate SOCS3/JAK1/STAT3 signaling pathway in mice with chronic obstructive pulmonary disease].
Zhen Ci Yan Jiu
December 2024
College of Acupuncture-moxibustion and Tuina, Anhui University of Chinese Medicine, Hefei 230038, China.
Objectives: To explore whether electroacupuncture(EA) can increase the expression of suppressor of cytokine signaling (SOCS)3 by affecting the expression of miR-19b-3p, inhibiting the continuous activation of janus kinase (JAK)1 /signal transducer and activator of transcription (STAT)3 signaling pathway, and improve pulmonary inflammation in chronic obstructive pulmonary disease (COPD) mice.
Methods: Forty mice were randomly divided into normal, COPD model, COPD+EA, and COPD+miR-19b-3p agomir (agomir)+EA groups. The COPD model was simulated by cigarette smoke exposure for 1 h, twice a day for 3 months.
Background: Hundreds of miRNAs have been reported to be dysregulated in Parkinson's disease (PD), providing valuable assistance in improving its diagnosis. The purpose of this study was to analyze the expression and diagnostic value of miR-19b-3p in PD patients, as well as its relationship with inflammatory factors.
Methods: We recruited 50 PD patients and 50 healthy age- and gender-matched controls and collected demographic data and biochemical parameters from both groups.
MiR-19b-3p inhibits cell viability and proliferation and promotes apoptosis by targeting IGF1 in KGN cells.
Gynecol Endocrinol
December 2024
Department of Reproductive Medicine, the First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, Fujian, China.
Background: Endometriosis (EM) is a major cause of infertility, but the pathogenesis and mechanisms are not yet fully elucidated. MiR-19b-3p is involved in many diseases, but its functional role in EM-associated infertility remains unexplored. This study aimed to examine miR-19b-3p abundance and IGF1 concentration in cumulus cells (CCs) and follicular fluid of EM-associated infertility patients, and to investigate the potential role of miR-19b-3p in KGN cells by identifying its target and elucidating the underlying mechanisms.
View Article and Find Full Text PDFCircYTHDF1/miR-19b-3p/YTHDF1 axis contributes to pregnancy-induced hypertension development by enhancing vascular endothelial cell injury.
Hypertens Pregnancy
December 2024
Department of Obstetrics and Gynecology, Fujian Maternity and Child Health Hospital, College of Clinical Medical for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, People's Republic of China.
Objective: The biological role of circ_0004858 (circYTHDF1) in pregnancy-induced hypertension (PIH) and the underlying mechanisms were unknown, and which were explored in this study.
Methods: ELISA was employed to detect the level of inflammatory cytokines and biochemical parameters; flow cytometry was employed to detect cell apoptosis; western blot and qRT-PCR were employed to examine expression level.
Results: The level of IL-1β, TNF-α, IL-6, TGF-β1, ET-1, and Ang-II were significantly elevated in the peripheral blood of PIH patients.
Transcriptomic Profiling of Primary Microglia: Effects of miR-19a-3p and miR-19b-3p on Microglia Activation.
Int J Mol Sci
October 2024
Department of Orthopaedics, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Neuropathic pain resulting from spinal cord injury (SCI) is a significant secondary health issue affecting around 60% of individuals with SCI. After SCI, activation of microglia, the immune cells within the central nervous system, leads to neuroinflammation by producing pro-inflammatory cytokines and affects neuropathic pain. This interplay between inflammation and pain contributes to the persistent and intense pain experienced by many individuals with SCI.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!