Rice bacterial blight is a devastating disease worldwide, causing significant yield losses. Understanding how plants defend against microbial infection is critical for sustainable crop production. We previously identified a pathogen-induced long noncoding RNA (ALEX1). In this study, we show that ALEX1 localizes to the nucleus and directly binds to AUXIN RESPONSE FACTOR 3 (ARF3). We demonstrate that ARF3 forms condensates in the nucleus via its intrinsically disordered middle region (MR). Notably, ARF3 condensates takes on solid-like properties. We further revealed that ALEX1 directly binds to the MR of ARF3, regulating ARF3 condensate dynamics and promotes ARF3 homodimerization. The dispersed and dimeric form of ARF3, referred to as its functional phase state, enhances its ability to transcriptionally repress downstream target gene such as JAZ13, thereby modulating the jasmonic acid (JA) signaling pathway and strengthening pathogen resistance in rice. This study highlights the role of a long noncoding RNA in regulating protein condensation and assembly, contributing to pathogen defense in plants.
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http://dx.doi.org/10.1016/j.molp.2024.12.005 | DOI Listing |
Environ Toxicol Pharmacol
December 2024
Center for Clinical Single-Cell Biomedicine, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou450003, Henan, China. Electronic address:
Cleft palate is the most prevalent congenital condition. Cleft palate is brought on by an exogenous chemical called all-trans retinoic acid (atRA). In order to indirectly control gene expression, long chain non-coding RNAs (lncRNAs) act as competitive endogenous RNA (ceRNA) sponges.
View Article and Find Full Text PDFBMC Cancer
December 2024
Department of Cardiothoracic Surgery, Shenzhen Guangming District People's Hospital, Shenzhen, China.
Background: Long non-coding RNA 01116 (linc01116) has been shown to be dysregulated in many tumors, and is closely related to the prognosis. This meta-analysis aimed to examine the correlation between linc01116 expression and cancer prognosis.
Methods: Six electronic databases were searched, and eligible studies were screened based on the inclusion and exclusion criteria.
Metabolism
December 2024
Department of Pathology, School of Basic Medical Sciences, Wannan Medical College, Wuhu, China; Postdoctoral Research Station of Clinical Medicine, Jinan University, Guangzhou, China. Electronic address:
Background & Aims: Abnormal expression of long noncoding RNAs is strongly linked to metabolic dysfunction-associated steatotic liver disease (MASLD). However, the precise molecular mechanisms remain unclear. This study explores the roles of noncoding RNA activated by DNA damage (NORAD)/miR-511-3p/Rho-associated protein kinase 2 (Rock2) axis and the NORAD/ROCK2 interaction in the development of MASLD.
View Article and Find Full Text PDFPlant Commun
December 2024
Jiangsu International Joint Center of Genomics, Jiangsu Key Laboratory of Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu Province 221116, China,. Electronic address:
Epitranscriptomic chemical modifications of RNAs have emerged as potent regulatory mechanisms in the plant stress adaptation process. Currently, over 170 distinct chemical modifications have been identified in mRNAs, tRNAs, rRNAs, microRNAs (miRNAs), and long-noncoding RNAs (lncRNAs). The genetic and molecular studies have identified the genes responsible for adding and removing chemical modifications on RNA molecules, known as "writers" and "erasers," respectively.
View Article and Find Full Text PDFMol Neurodegener
December 2024
Department of Neuroscience, Mayo Clinic, Jacksonville, FL, USA.
Background: The gene C9orf72 harbors a non-coding hexanucleotide repeat expansion known to cause amyotrophic lateral sclerosis and frontotemporal dementia. While previous studies have estimated the length of this repeat expansion in multiple tissues, technological limitations have impeded researchers from exploring additional features, such as methylation levels.
Methods: We aimed to characterize C9orf72 repeat expansions using a targeted, amplification-free long-read sequencing method.
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