AI Article Synopsis
- - The study investigates hub genes linked to central post-stroke pain (CPSP) in ischemic stroke (IS) using public databases and bioinformatics to enhance understanding of CPSP in IS patients.
- - Researchers identified 13 differentially expressed genes related to significant pathways and narrowed it down to 3 hub genes (CD163, MMP9, ARG1) that are highly expressed in IS patients and show promising diagnostic capabilities.
- - The study also explored various immune cell infiltration differences, predicted relevant microRNAs and transcription factors, and identified potential small molecular drugs, thus providing insights into the mechanisms of CPSP in IS and laying groundwork for future research.
Article Abstract
This investigation aims to screen ischemic stroke (IS)-related hub genes of central post-stroke pain (CPSP) from public databases and predict their potential roles through bioinformatics analysis to better interpret CPSP in IS. First, based on differential analysis, Venn analysis, and enrichment analyses, we identified 13 differently expressed genes in CPSP (CPSP-DEGs) related to the TNF signaling pathway, Vascular smooth muscle contraction, and IL-17 signaling pathway. Subsequently, through screening and analysis of the PPI network constructed by the Search Tool for the Retrieval of Interacting Genes (STRING) database, we obtained 3 CPSP-related hub genes (CD163, MMP9, and ARG1). They were all highly expressed in the IS group, exhibiting good diagnostic performance, with area under curve (AUC) value > 0.85. The immune-related analysis demonstrated that the infiltration levels of various immune cells in the IS group and the normal group were substantially different. In addition, by utilizing some online websites, we not only predicted some microRNAs (miRNAs) and transcription factors (TFs) that may target hub genes but also mined small molecular drugs that may target differentially expressed genes (DEGs) in IS. In conclusion, this project first investigated the role of CPSP-related genes in IS and identified 3 hub genes. At the same time, we predicted some miRNAs, TFs, and candidate drugs that may target hub genes. Our research uncovered the potential mechanism of CPSP-related genes in IS from multiple perspectives. Furthermore, it also laid a research foundation for the future study of the mechanisms of IS disease.
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| http://dx.doi.org/10.1093/hmg/ddae178 | DOI Listing |
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