Role of COX6C and NDUFB3 in septic shock and stroke.

Background: Septic shock is a clinical syndrome characterized by acute circulatory disturbance. Stroke is an acute cerebrovascular disease caused by brain tissue damage. However, the relationship of COX6C and NDUFB3 to them is unclear.

Method: The stroke dataset GSE58294 and the septic shock dataset GSE15491 were downloaded from the gene expression omnibus database. Screening of differentially expressed genes (DEGs), weighted gene co-expression network analysis, construction and analysis of protein-protein interaction network, functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis, and comparative toxicogenomics database (CTD) analysis were performed. Gene expression heat map was drawn. TargetScan screened miRNAs regulating central DEGs.

Results: A total of 664 DEGs were obtained. Gene ontology analysis showed that they were mainly enriched in leukocyte activation, intracellular vesicle, neutrophil activation, and cytokine receptor activity. According to Kyoto Encyclopedia of Genes and Genomes analysis, they are mainly enriched in metabolic pathways, phagosomes, and infection. Core genes (UQCRQ, USMG5 [ATP5MD], COX6C, NDUFB3, ATP5L [ATP5MG], COX7C, NDUFA1, NDUFA4) were highly expressed in septic shock and stroke samples. CTD analysis found that eight core genes are associated with liver enlargement, inflammation, proliferation, fibrosis, and necrosis.

Conclusion: COX6C and NDUFB3 genes are highly expressed in septic shock and stroke. The higher the COX6C and NDUFB3 genes, the worse the prognosis.