Development of an automatic tubular chemiluminescence immunoassay using magnetic particles for the detection of antibodies against foot-and-mouth disease virus serotype A.

Microb Pathog

Lanzhou Shouyan Biotechnology Co., Ltd, Lanzhou, 730046, China; State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-Mouth Diseases Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. Electronic address:

Published: December 2024

Foot-and-mouth disease virus (FMDV) causes significant financial losses in animal farms worldwide. Specific antibody monitoring of FMDV post-vaccination is important for evaluating vaccine efficacy, as well as the prevention and control of foot-and-mouth disease (FMD). In this study, we developed a fully automatic tubular chemiluminescence immunoassay method based on magnetic particles (A-MPCLIA) to specifically detect antibodies against FMDV serotype A. The diagnostic sensitivity (Dsn) and diagnostic specificity (Dsp) of A-MPCLIA were determined to be 96.05 % and 99 %, respectively, with a cut-off value of 30 activity units (U) by detecting the known background swine, cattle, and sheep serum. The developed method presented good Dsn, Dsp, and repeatability (CV < 10 %). The activity units of A-MPCLIA, determined using 23 animals vaccinated with FMDV serotypes O, A, and Asia 1 univalent inactivated vaccine and nine serum samples from naïve animals, were significantly positively correlated with the titers of liquid-phase-blocking enzyme-linked immunosorbent assay (R = 0.992) and the titer of the virus neutralization test (R = 0.9577). In addition, A-MPCLIA requires less than 20 min, and it is fully automatic in detection. These results showed that A-MPCLIA is a suitable method for the detection of antibodies against FMDV serotype A in the future.

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Source
http://dx.doi.org/10.1016/j.micpath.2024.107211DOI Listing

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