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Ptychognathus Stimpson, 1858 is a speciose genus in the family Varunidae, widely distributed in the Indo-West Pacific. Although species of Ptychognathus are common in estuaries with low salinity, some species are difficult to be identified correctly because of their small body size and similar morphology. This study describes one new species, Ptychognathus amikee sp.

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In the present contribution, five species of Ptychognathus Stimpson, 1858 are reported from Polynesia: P. crassimanus Finnegan, 1931, P. easteranus Rathbun, 1907, P.

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Examination of material from various Japanese localities clarified that the estuarine crab Ptychognathus glaber Stimpson, 1858 is possibly restricted to the Ogasawara Islands, Japan. Specimens from the Ryukyu Islands, thought to represent P. glaber, actually represent P.

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Species in the brackish crab genus are common in the seashore and estuary habitats with freshwater input. Due to their similar morphologies and dull colorations, it is always difficult to distinguish the species in this genus. In this study, the DNA barcode gene (cytochrome oxidase subunit I) was used to help identify from Taiwan.

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