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Zinc finger protein rotund is essential for wings and ovarian development by regulating lipid homeostasis in Locusta migratoria. | LitMetric
Cys2-His2-type zinc finger (C2H2-ZF) proteins are involved in diverse biological processes. In insects, the wing and ovarian development is crucial for reproduction and evolution, yet the physiological roles of C2H2-ZF proteins in these processes remain underexplored. Here, RNA-seq analyses identified C2H2-ZF protein genes that were differentially expressed during wing formation in Locusta migratoria. Among these, the gene encoding a C2H2-ZF protein Rotund (Rn) was highly expressed in the wing pads of fourth- and fifth-instar nymphs. RNA interference mediated knockdown of LmRn in nymph stages resulted in pronounced abnormalities with curled wings and reduced wing area. LmRn knockdown led to reduced expression of lipid transport-related genes during wing morphogenesis, significantly decreased triglyceride (TG) level. In addition, we also find that LmRn knockdown impaired ovarian development and oocyte maturation in female adults, with decreased expression levels of lipid synthesis-related genes, and vitellogenin genes (VgA, and VgB) in the fat body. Meanwhile, the number of lipid droplets, TG content, and protein levels in the ovaries were significantly decreased after LmRn was silenced. Together, our findings reveal that LmRn is essential for wing and ovarian development by regulating lipid homeostasis in locusts, offering a potential target for insect pest management.
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