AI Article Synopsis
- A circuit-based gene therapy has effectively reversed key symptoms of Parkinson's disease in animal models, including mice and primates.
- This approach targets dopamine receptor D-expressing neurons through a combination of adeno-associated virus (AAV) delivery and chemogenetic techniques.
- The research includes a step-by-step guide for AAV injection, creating PD models in primates, and evaluating the therapy's impact on motor symptoms.
Article Abstract
A circuit-based gene therapy strategy for Parkinson's disease (PD) has been shown to significantly reverse core symptoms in both murine and primate PD models. Here, we present a comprehensive workflow to specifically manipulate dopamine receptor D-expressing medium spiny neurons by retrograde adeno-associated virus (AAV) transduction and chemogenetic activation using a designer toolkit. We describe steps for AAV injections and PD primate model induction. We then detail behavioral measurements to assess the therapeutic efficacy of the therapy for motor symptoms.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656076 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103470 | DOI Listing |
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