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Exploring Cd tolerance and detoxification strategies of ssp. under high cadmium exposure.
Int J Phytoremediation
January 2025
Graduate School of Environmental Studies, Tohoku University, Sendai, Japan.
ssp. is well known as a Cd hyperaccumulator. Yet, understanding how this plant survives in a high Cd environment without appearing toxicity signs is far from complete.
View Article and Find Full Text PDFSub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3.
Nat Commun
January 2025
Neuroscience Institute, New York University Langone Health, New York, NY, 10016, USA.
Apical and basal dendrites of pyramidal neurons receive anatomically and functionally distinct inputs, implying compartment-level functional diversity during behavior. To test this, we imaged in vivo calcium signals from soma, apical dendrites, and basal dendrites in mouse hippocampal CA3 pyramidal neurons during head-fixed navigation. To capture compartment-specific population dynamics, we developed computational tools to automatically segment dendrites and extract accurate fluorescence traces from densely labeled neurons.
View Article and Find Full Text PDFSpatioselective Imaging of Noncoding RNAs in Mitochondria via an Organelle-Specific DNA Assembly Strategy.
Nano Lett
January 2025
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.
Precise imaging of noncoding RNAs (ncRNAs) in specific organelles allows decoding of their functions at subcellular level but lacks advanced tools. Here we present a DNA-based nanobiotechnology for spatially selective imaging of ncRNA (e.g.
View Article and Find Full Text PDFCellSP: Module discovery and visualization for subcellular spatial transcriptomics data.
bioRxiv
January 2025
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Spatially resolved transcriptomics has made it possible to study the subcellular organization of mRNA, a critical aspect of cellular function. However, there is a dearth of analytical tools to identify and interpret the functional significance of subcellular spatial distribution patterns. To address this, we present CellSP, a computational framework for identifying, visualizing, and characterizing consistent subcellular spatial patterns of mRNA.
View Article and Find Full Text PDFImaging cells and nanoparticles using modulated optically computed phase microscopy.
Sci Rep
January 2025
Department of Chemistry & Environmental Science, Jordan Hu College of Science and Liberal Arts, New Jersey Institute of Technology, Newark, NJ, 07102, USA.
Nanoparticles (NPs) have been successfully used as drug delivery systems. To develop and optimize NP-based drug delivery systems, it is essential to understand the dynamics of cell-NP interactions. Quantitative phase imaging techniques enable label-free imaging and have the potential to reveal how cells interact with NPs.
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