Inhalation of silica dust in the workplace has been addressed as a serious occupational pulmonary disease subsequently leading to inflammation and fibrosis. Enhanced expression of IL-10 significantly contributes to the disease etiology, along with an elevated Th2-type paradigm. Previously, we showed that the exaggerated Th2-type response was also associated with consistent upregulation of Stat3 in mouse airways stimulated with silica microparticles. However, a precise understanding of silicosis in light of the IL-10/Stat3 immune axis is required. We, therefore, aimed to determine the regulatory role of IL-10 in nanosized silica (nSiO)-induced pulmonary fibrosis in association with Stat3. Herein, we report that amorphous nSiO could induce pulmonary fibrosis with consistent and concomitant upregulation of IL-10, Stat3, and SR-A/CD204. Following exogenous administration of siStat3 and rIL-10, the study further confirmed that Stat3 mediates the regulation of IL-10 and SR-A/CD204 and that IL-10 could regulate its own expression in an autoregulatory loop. The ChIP assay highlighted the localization of Stat3 over two putative binding sites in the IL-10 promoter region, which subsequently resulted in the overexpression of SR-A/CD204. Conclusively, Stat3-mediated transregulation of IL-10 through an autoregulatory loop in silicosis could offer novel molecular targets for therapeutic interventions.
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http://dx.doi.org/10.1021/acsbiomaterials.4c01473 | DOI Listing |
Microb Cell Fact
January 2025
Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
Carbapenem-resistant Klebsiella pneumoniae poses a severe risk to global public health, necessitating the immediate development of novel therapeutic strategies. The current study aimed to investigate the effectiveness of the green algae Arthrospira maxima (commercially known as Spirulina) both in vitro and in vivo against carbapenem-resistant K. pneumoniae.
View Article and Find Full Text PDFJ Cyst Fibros
January 2025
Cystic Fibrosis Therapeutics Development Network Coordinating Center, Seattle Children's Hospital, Seattle, WA, USA; Department of Pediatrics, University of Washington, Seattle, WA, USA; Department of Biostatistics, University of Washington, Seattle, WA, USA.
Background: Highly effective CFTR modulators improve CFTR function and lead to dramatic improvements in health outcomes in many people with cystic fibrosis (pwCF). The relationship between measures of CFTR function, such as sweat chloride concentration, and clinical outcomes in pwCF treated with CFTR modulators is poorly defined. We conducted analyses to better understand the relationships between sweat chloride and CFTR function in vitro, and between sweat chloride and clinical outcomes following CFTR modulator treatment.
View Article and Find Full Text PDFRespir Med
January 2025
Division of Pulmonology, Department of Internal Medicine, Myongji Hospital, Hanyang University College of Medicine, Gyeonggi-do, Republic of Korea. Electronic address:
Introduction: Ca2+ signaling in fibroblasts would be one of the important mediators of lung fibrosis. This study investigated the relationship between calcium channel blocker usage and the risk of developing interstitial lung disease and idiopathic pulmonary fibrosis.
Material And Methods: This cohort study used data from the Korean National Health Screening Cohort spanned from January 1, 2004, to December 31, 2015.
Life Sci
January 2025
Department of Biological Sciences (Regulatory Toxicology), National Institute of Pharmaceutical Education and Research (NIPER), Balanagar, Hyderabad, TS 500037, India. Electronic address:
Pulmonary fibrosis (PF) arises from dysregulated wound healing, leading to excessive extracellular matrix (ECM) deposition and impaired lung function. Macrophages exhibit high plasticity, polarizing to pro-inflammatory M1 during early inflammation and anti-inflammatory, fibrosis-inducing M2 during later stages of PF. Additionally, neutrophils and neutrophil extracellular traps (NETs) release mediated by peptidyl arginine deiminase (PAD-4), also play a key role in PF progression.
View Article and Find Full Text PDFFood Chem Toxicol
January 2025
Department of Histology and Embryology, Erciyes University, Faculty of Medicine, 38039 Kayseri, Turkey. Electronic address:
Favipiravir, a broad-spectrum RNA-dependent RNA polymerase inhibitor widely used during the COVID-19 pandemic, effectively reduces viral load but has been linked to inflammatory changes in tissues such as the liver and kidneys. High-dose and prolonged use of favipiravir for COVID-19 raises concerns about its potential toxic effects on the lungs, particularly in patients with pre-existing pulmonary conditions. This study investigated favipiravir's effects on lung tissue in healthy rats.
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