Traumatic brain injury (TBI) induces neurogenerative disorders affecting severely daily human activities and early diagnosis is a critical requirement for prevention and cure. Here, we induced TBI formation in the Zebra fish, a model organism, by both mechanical (ultrasonic cleaner generated convulsive shock, UGCS) and chemical (pentylenetetrazol, PTZ) methods. The TBI induced cellular and neuronal changes are monitored by measuring the activities of the indicator biomarkers viz., superoxide anion (O) and glutamate by electrochemical techniques. For this, the α-lipoic acid (α-LA, LA) functionalized gold-silver (LA-Au/Ag) is used as an electrochemical sensor to diagnose the presence of these markers in physiological phosphate buffer saline (PBS, pH 7.4), 0.1 M KCl solutions and in TBI tissues. While the oxidation of glutamate is observed in the potential window 0.2-0.5 V, the metal mediated oxidation of O is observed at the potential window 0.6-1.0 V. The sensor showed good linear ranges for O (from 4 to 48 μM with the LOD of 4 μM for the O detection) and glutamate (from 20 to 130 μM with the LOD 19 μM). The TBI tissue modified electrode showed lower resistance than the normal brain tissue ((NBT), as control) due to the presence of higher amount of O and occurrence of Fenton's and Heber-Weise's reactions in the presence of [Fe(CN)]. For theragnostic application, the LA-Au/Ag nanoparticles is delivered into the UGCS and PTZ treated Zebrafish and electrochemical signal changes are monitored by cyclic voltammetry and impedance spectroscopy. Electrochemical data further corroborated with the activities of superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidase (MDA) in parallel. The developed method of electrochemical sensing of TBI may provide alternative for the early TBI diagnosis and therapeutics for the prevention of TBI.
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http://dx.doi.org/10.1016/j.bioelechem.2024.108871 | DOI Listing |
Eur J Pediatr
December 2024
Institute of Psychology, Faculty of Psychology and Sport Science, University of Innsbruck, Innsbruck, Austria.
The present study aims at providing reference values from the general pediatric population for the German version of the 21-item self-report post version of the Postconcussion Symptom Inventory for adolescents aged 13-17 years (PCSI-SR13) following pediatric traumatic brain injury (pTBI). A total of N = 950 adolescents completed an adapted version of the PCSI-SR13. Prior to establishing reference values using percentiles, psychometric properties (i.
View Article and Find Full Text PDFPhys Sportsmed
December 2024
Department of Kinesiology and Applied Physiology, University of Delaware, Newark, DE, USA.
Objectives: To cross-sectionally determine 1) the association between lifetime diagnosed concussion and upper extremity musculoskeletal injury (UE-MSI) amongst a novel cohort of community rugby union players and 2) the sex specific risk of UE-MSI given concussion history among these rugby players.
Methods: 1,037 (31.0% female, 31.
Acid-sensing ion channels (ASICs) are typically activated by acidic environments and contribute to nociception and synaptic plasticity. ASIC1a is the most abundant subunit in the central nervous system and forms homomeric channels permeable to Na and Ca , making it a compelling therapeutic target for acidotic pathologies including stroke and traumatic brain injury. However, a complete conformational library of human ASIC1a in its various functional states has yet to be described.
View Article and Find Full Text PDFBrain Commun
December 2024
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Columbia University, New York, NY 10027, USA.
Magnetic resonance elastography has emerged over the last two decades as a non-invasive method for quantitatively measuring the mechanical properties of the brain. Since the inception of the technology, brain stiffness has been the primary metric used to describe brain microstructural mechanics. However, more recently, a secondary measure has emerged as both theoretical and experimental significance, which is the ratio of tissue viscosity relative to tissue elasticity.
View Article and Find Full Text PDFRisk Manag Healthc Policy
December 2024
Department of Trauma Surgery, Emergency Surgery & Surgical Critical, Tongji Trauma Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Background: Deep venous thrombosis (DVT), known to be a major factor in poor outcomes and death rates, is common after polytrauma with traumatic brain injury (TBI). In this study, a nomogram will be developed to predict the risk of DVT in polytrauma patients with TBI, since there is currently no specific and convenient diagnostic method.
Methods: A retrospective and observational trial was conducted between November 2021 and May 2023.
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