Non-contact exposure to dinotefuran disrupts honey bee homing by altering MagR and Cry2 gene expression.

Dinotefuran is known to negatively affect honeybee (Apis mellifera) behavior, but the underlying mechanism remains unclear. The magnetoreceptor (MagR, which responds to magnetic fields) and cryptochrome (Cry2, which is sensitive to light) genes are considered to play important roles in honey bees' homing and localization behaviors. Our study found that dinotefuran, even without direct contact, can act like a magnet, significantly altering MagR expression in honeybees. This non-contact exposure reduced the bees' homing rate. In further experiments, we exposed foragers to light and magnetic fields, the MagR gene responded to magnetic fields only in the presence of light, with Cry2 playing a key switching role in the magnetic field receptor mechanism (MagR-Cry2). Yeast two-hybrid and BiFc assays confirmed an interaction of these two genes. Moreover, the bees' homing rate was significantly reduced when the expression of these genes was decreased using RNAi. These findings suggest that changes in MagR and Cry2 expression are critical to the reduction in homing ability caused by non-contact dinotefuran exposure. This study reveals the potential navigation mechanisms of honey bees during homing and foraging and shows that the impact of dinotefuran on honey bee populations is more extensive than previously understood.