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A novel ratiometric BenzoBODIPY-Based fluorescent probe for the detection and imaging of Cysteine in living cells and zebrafish models. | LitMetric
Cysteine (Cys) plays a critical role in various biological processes, including protein synthesis, cellular signaling, and antioxidant defense. However, precise detection of Cys in biological systems remains challenging due to interference from similar thiols such as homocysteine (Hcy) and glutathione (GSH). In this study, we report the synthesis and bioimaging of a novel ratio-type fluorescent probe based on the benzoBODIPY fluorophore, designed for the ratiometric detection of Cys. The probe operates through an intramolecular charge transfer (ICT) mechanism, where the reaction with Cys triggers a substitution reaction with 4-mercaptopyridine, followed by a Smiles rearrangement. This results in a shift from red to yellow-green fluorescence, providing a sensitive and specific method for the quantitative detection of Cys. The probe demonstrates excellent selectivity, with significantly lower responses to Hcy and GSH, and has been successfully applied in bioimaging experiments in HeLa cells and zebrafish models, highlighting its potential for diagnosing and treating Cys-related diseases.
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