AI Article Synopsis
- An ideal treatment for type-1 diabetes should ideally stop the death of insulin-producing beta cells and increase their numbers.
- The study examined how combining the BCG vaccine with liraglutide and probiotics could help diabetic rats by reducing harmful signaling pathways triggered by a diabetes-inducing agent.
- Results showed that this combination significantly improved blood sugar levels, reduced inflammation and cell death in the pancreas, and enhanced overall insulin production and function.
Article Abstract
Background: An ideal anti-diabetic type-1 pharmacotherapy should combine abrogation of beta cell pyroptosis with enhancement of beta cell mass.
Objectives: The study investigated the potential synergism from combining the Bacillus Calmette-Guerin (BCG) vaccine with liraglutide (LIR) and probiotics in mitigating Streptozocin (STZ)-induced Type1diabetes mellitus in albino rats suppression of TXNIP/NLRP3 signaling. Induction of diabetes was performed by two I.V. injections of 50 mg/kg of STZ in male Wistar rats. Forty-eight rats were randomly allocated into six groups: Normal control group; STZ -diabetic group; BCG group; BCG + LIR group; BCG + probiotic group; BCG + LIR + probiotic group. The rats were sacrificed after 8 weeks of treatment.
Results: The STZ-diabetic group exhibited significant elevation of fasting blood sugar and HbA1c with remarkably decreased serum insulin along with a considerable increase in pancreatic proinflammatory cytokines (TNF-α, NLRP3, IL-1β, and NFκB) and apoptotic markers (ASK-1, IAPP, TXNIP, and Caspase-3) with prominently compromised oxidative scavenging capacity in addition to structural alteration in the pancreatic histoarchitecture with decreased insulin immunostaining. Conversely, diabetic-treated groups, especially the BCG + LIR + probiotic group, were superior in amelioration of STZ-induced pyroptosis of pancreatic islets evidenced by a significant decline in inflammatory cytokines and apoptotic markers with a remarkable upgrade in redox balance, Furthermore, the mitigation in the altered histopathological picture of the pancreas with enhanced insulin immunostaining has been was mirrored on the significant improvement of glucose homeostasis parameters.
Conclusions: Noteworthy, BCG combination with liraglutide and probiotic might be a promising repurposed therapeutic modality in the management of type-1 diabetes mellites targeting pancreatic TXNIP/NLRP3 signaling pathway.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11620097 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2024.e38932 | DOI Listing |
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