The SLR1-OsMADS23-D14 module mediates the crosstalk between strigolactone and gibberellin signaling to control rice tillering.

Strigolactones (SLs) and gibberellins (GAs) have been found to inhibit plant branching or tillering, but molecular mechanisms underlying the interplay between SL and GA signaling to modulate tillering remain elusive. We found that the transcription factor OsMADS23 plays a crucial role in the crosslink between SL and GA signaling in rice tillering. Loss-of-function mutant osmads23 shows normal axillary bud formation but defective bud outgrowth, thus reducing the tiller number in rice, whereas overexpression of OsMADS23 significantly increases tillering by promoting tiller bud outgrowth. OsMADS23 physically interacts with DELLA protein SLENDER RICE1 (SLR1), and the interaction reciprocally stabilizes each other. Genetic evidence showed that SLR1 is required for OsMADS23 to control rice tillering. OsMADS23 acts as an upstream transcriptional repressor to inhibit the expression of SL receptor gene DWARF14 (D14), and addition of SLR1 further enhances OsMADS23-mediated transcriptional repression of D14, indicating that D14 is the downstream target gene of OsMADS23-SLR1 complex. Moreover, application of exogenous SL and GA reduces the protein stability of OsMADS23-SLR1 complex and promotes D14 expression. Our results revealed that SLs and GAs synergistically inhibit rice tillering by destabilizing OsMADS23-SLR1 complex, which provides important insights into the molecular networks of SL-GA synergistic interaction during rice tillering.