The World Health Organization set a goal of reducing salt intake by 30 % by 2025. This study investigates the impact of replacing 33 % NaCl with KCl and microencapsulated spices and aromatic plant extracts (ME) in a dry-cured meat sausage (CMS). Microbial, physico-chemical, and sensory analyses were conducted throughout processing and storage. Three batches of CMS were prepared with four formulations: Control (1.5 % NaCl), F1 (1 % NaCl, 0.5 % KCl), F2 (1 % NaCl, 0.5 % ME, 0.3 % KCl), and F3 (1 % NaCl, 0.5 % ME). The absence of Listeria monocytogenes was confirmed. The formulations did not affect the growth of lactic acid bacteria (7.8 log cfu/g), Enterococci (6.5 log cfu/g), and coagulase-negative staphylococci (5.6 log cfu/g). Biogenic amines increased significantly (P < 0.05) during storage, with cadaverine (from 166 to 456 mg/kg), tyramine (163 to 424 mg/kg) and putrescine (from 31.0 to 90.5 mg/kg), being the most abundant. All low sodium CMS had lower TBARS values (F1 = 0.59 mg MDA/kg, F2 = 0.56 mg MDA/kg and F3 = 0.47 mg MDA/kg) compared to control (0.78 mg MDA/kg). Colour parameters lightness (L*) and yellowness (b*) remained stable (P > 0.05) while sausages with KCl and/or ME were redder. CMS F1 was considered with the ideal saltiness by 54 % consumers, that is usually considered enough to launch the product in the market. The use of ME in CMS has potential but still requires optimization. The study demonstrates that a 33 % NaCl replacement with KCl is feasible without jeopardize the organoleptic characteristics or safety of CMS.
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http://dx.doi.org/10.1016/j.meatsci.2024.109719 | DOI Listing |
ACS Infect Dis
May 2016
Mycobacterial Metabolism and Antibiotic Research Laboratory and Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, Mill Hill Laboratory, London NW7 1AA, United Kingdom.
The mechanisms that lead to phenotypic antibacterial tolerance in bacteria remain poorly understood. We investigate whether changes in NaCl concentration toward physiologically higher values affect antibacterial efficacy against (Mtb), the causal agent of human tuberculosis. Indeed, multiclass phenotypic antibacterial tolerance is observed during Mtb growth in physiologic saline.
View Article and Find Full Text PDFBiochemistry
February 2010
MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
A single-stranded DNA binding protein (SSB), labeled with a fluorophore, interacts with single-stranded DNA (ssDNA), giving a 6-fold increase in fluorescence. The labeled protein is the adduct of the G26C mutant of the homotetrameric SSB from Escherichia coli and a diethylaminocoumarin {N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide}. This adduct can be used to assay production of ssDNA during separation of double-stranded DNA by helicases.
View Article and Find Full Text PDFInorg Chem
September 2002
Department of Chemistry, The University of Iowa, Iowa City, IA 52242, USA.
Reduction of TaCl(5) with either Ga or Ga(2)Cl(4), in the presence of NaCl, in a sealed borosilicate glass ampule at 500 degrees C, followed by aqueous Soxhlet extraction and treatment with SnCl(2) and hydrochloric acid, yielded Ta(6)(mu-Cl)(12)Cl(2)(OH(2))(4).4H(2)O in 92% (Ga) or 96% (Ga(2)Cl(4)) yield. Ga(2)Cl(4), a probable intermediate in the Ga-based reduction, is a more convenient reductant than Ga because it is readily dispersed in the reaction mixture, and these mixtures do not require homogenizations in order to afford high yields.
View Article and Find Full Text PDFJ Neuroendocrinol
April 1990
Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK.
Abstract In cats anaesthetized with intravenous chloralose, the injection of 0.05 to 0.4 ml 1.
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