Proximate analysis and profiles of amino acids, fatty acids, and minerals in insect-based foods for dogs.

Objective: To analyze crude protein, crude fat, crude ash, crude fiber, amino acids, fatty acids, and minerals in insect-based dog foods and to evaluate their compliance with nutritional guidelines.

Methods: Proximate analysis, mineral analysis, amino acid profiling, and fatty acid composition analysis were conducted from November 27, 2023, through February 2024 on 18 commercially available insect-based dog foods formulated for all-life-stage or adult dogs.

Results: Proximate analysis results revealed that all 18 pet foods met the Association of American Feed Control Officials guidelines. However, discrepancies were observed between the values listed on the packaging and those measured in 7 foods. Mineral analysis showed that while all foods met the Association of American Feed Control Officials guidelines for magnesium, discrepancies were found in calcium, phosphorus, zinc, iron, and copper content, with several samples failing to meet recommended levels. Additionally, 2 foods exceeded the recommended maximum ratio for certain fatty acids. Black soldier fly larvae-based foods contained higher levels of lauric and myristic acids compared to other insect-based foods.

Conclusions: Insect-based dog foods show promise as sustainable protein sources, but discrepancies in mineral content and fatty acid ratios highlight the need for both stricter regulation and better enforcement of existing guidelines to ensure nutritional adequacy for dog health and accurate labeling.

Clinical Relevance: This study provides valuable insights into the nutritional composition of insect-based dog foods, revealing inconsistencies in mineral content and fatty acid ratios. These findings can help the pet food industry develop more nutritionally consistent insect-based diets.