[This corrects the article DOI: 10.1371/journal.pone.0279834.].
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11620568 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0315421 | PLOS |
mSystems
January 2025
Department of Infectious Diseases, School of Translational Medicine, Monash University, Melbourne, Victoria, Australia.
Unlabelled: Sequencing DNA directly from patient samples enables faster pathogen characterization compared to traditional culture-based approaches, but often yields insufficient sequence data for effective downstream analysis. CRISPR-Cas9 enrichment is designed to improve the yield of low abundance sequences but has not been thoroughly explored with Oxford Nanopore Technologies (ONT) for use in clinical bacterial epidemiology. We designed CRISPR-Cas9 guide RNAs to enrich the human pathogen , by targeting multi-locus sequence type (MLST) and transfer RNA (tRNA) genes, as well as common antimicrobial resistance (AMR) genes and the resistance-associated integron gene .
View Article and Find Full Text PDFFoodborne Pathog Dis
January 2025
Department of Cardiology, The First Affiliated Hospital of Bengbu Medical University, Bengbu, China.
Listeriosis is a rare, life-threatening bacterial infection. Prompt, appropriate antibiotic treatment is essential to save lives. We aimed to investigate antibiotic selection and listeriosis knowledge among health care providers.
View Article and Find Full Text PDFNutrients
December 2024
Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-000, SP, Brazil.
The microbiota stability, diversity, and composition are pillars for an efficient and beneficial symbiotic relationship between its host and itself. Microbial dysbiosis, a condition where a homeostatic bacterial community is disturbed by acute or chronic events, is a predisposition for many diseases, including local and systemic inflammation that leads to metabolic syndrome, diabetes, and some types of cancers. Classical dysbiosis occurs in the large intestine.
View Article and Find Full Text PDFTalanta
January 2025
School of Pharmacy, Binzhou Medical University, Yantai, 264003, China. Electronic address:
Ciprofloxacin (CIP) is a commonly used antibiotic, but its abuse may cause bacterial resistance, posing a high risk to the environment and human health. Herein, based on the molecular imprinting technology, this study proposed a ratiometric fluorescence sensor employing the "post-doping" strategy, which aims to be rapid, selective, and visually easy-to-use for CIP detection to address antibiotic residues and environmental risks. Specifically, by exploiting the "antenna effect" of lanthanide metal ions (Ln), terbium (III) (Tb) chosen as a fluorescence-assisted functional monomer as well as the red emitting CdTe quantum dots (QDs) as the internal reference signal were introduced into multi-emission Tb-CdTe@SiO@MIPs (TbMIPs).
View Article and Find Full Text PDFBMC Ophthalmol
January 2025
Department of Tuberculosis, New District Branch of Northern Jiangsu People's Hospital of Jiangsu Province, Yangzhou, 225001, Jiangsu Province, China.
Background: This study aims to detect Mycobacterium tuberculosis complex (MTBC) DNA in intraocular fluid from clinically suspected tuberculous uveitis patients using multiplex polymerase chain reaction (PCR) and investigate the diagnostic utility of multiplex PCR for tuberculous uveitis.
Methods: Primers targeting three specific genes (MPB64, CYP141, and IS6110) within the MTBC genome were designed. Multiplex PCR was conducted using DNA from the H37Rv strain as well as DNA extracted from fluids of confirmed tuberculosis patients to assess primer specificity and method feasibility.
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